BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA

转化生长因子-β的生物学和分子生物学

基本信息

项目摘要

Three distinct isoforms of TGF-beta are expressed in mammals. Although each of these isoforms shows 65-85% homology to each other, the amino acid sequence of any particular isoform is conserved greater than 98% between mammalian, avian, and amphibian species. This strongly suggests that the mature peptides will have certain unique biological activities. Only TGF-beta's 1, and 2 have been purified from natural sources. So that we might be able to compare the activities of all three isoforms, we developed a system for expression of recombinant TGF-beta 3. Moreover, to begin to explore exactly which regions of the molecule are responsible for isoform-specific binding and activity, we have used this same expression system to express specific recombinant chimeric TGF-beta's in which regions of the amino acid sequence of TGF-beta's 1, 2, and 3 are substituted for each other in the mature peptide. Each of these recombinant TGF-beta's has been purified to homogeneity using a sequence of high pressure liquid chromatography steps. The availability of TGF-beta 3 has permitted us to raise antibodies against this peptide which can now be used to detect its presence in a variety of experimental systems. Moreover, selective effects of TGF-beta 3 have been identified in inhibition of the growth of hematopoietic cells, in migration of fetal fibroblasts, and in inhibiting the survival of certain neuronal cells. Using the chimeric TGF-beta constructs, we have been able to deduce that the middle third of the TGF-beta molecule is sufficient to confer isoform-specific biological activity. In a related aspect of this project, we are exploring the effects of the different TGF-beta isoforms on protection of cardiac myocytes from damage resulting from the action of growth factors such as interleukin-I and tumor necrosis factor-alpha.
三种不同的tgf - β亚型在哺乳动物中表达。虽然

项目成果

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A B ROBERTS其他文献

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{{ truncateString('A B ROBERTS', 18)}}的其他基金

STUDY OF MICE IN WHICH THE TGF BETA GENE HAS BEEN DISRUPTED
对 TGF Beta 基因被破坏的小鼠的研究
  • 批准号:
    2463689
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
STUDY OF MICE IN WHICH THE TGF BETA 1 GENE HAS BEEN DISRUPTED
对 TGF Beta 1 基因被破坏的小鼠的研究
  • 批准号:
    3752781
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
BIOLOGY AND RECEPTOR SIGNALLING OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和受体信号转导
  • 批准号:
    5201457
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
MECHANISM OF ACTION OF TYPE BETA TRANSFORMING GROWTH FACTOR
β型转化生长因子的作用机制
  • 批准号:
    4692310
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
NOVEL CHEMOPREVENTIVE AGENTS IN EXPERIMENTAL MAMMARY CARCINOGENESIS
乳腺癌实验中的新型化学预防剂
  • 批准号:
    6100875
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
  • 批准号:
    3838326
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
STRUCTURE/FUNCTION AND SIGNAL TRANSDUCTION PATHWAYS OF TGF BETA
TGF Beta 的结构/功能和信号转导途径
  • 批准号:
    6160869
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
NOVEL CHEMOPREVENTIVE AGENTS IN EXPERIMENTAL MAMMARY CARCINOGENESIS
乳腺癌实验中的新型化学预防剂
  • 批准号:
    6160975
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
  • 批准号:
    3916749
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
  • 批准号:
    3752614
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

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合作研究:DMS/NIGMS 1:使用由细胞内张力传感测量提供的多尺度 3D 模型模拟细胞迁移
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