BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
基本信息
- 批准号:3853409
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Three distinct isoforms of TGF-beta are expressed in mammals. Although
each of these isoforms shows 65-85% homology to each other, the amino
acid sequence of any particular isoform is conserved greater than 98%
between mammalian, avian, and amphibian species. This strongly suggests
that the mature peptides will have certain unique biological activities.
Only TGF-beta's 1, and 2 have been purified from natural sources. So
that we might be able to compare the activities of all three isoforms, we
developed a system for expression of recombinant TGF-beta 3. Moreover,
to begin to explore exactly which regions of the molecule are responsible
for isoform-specific binding and activity, we have used this same
expression system to express specific recombinant chimeric TGF-beta's in
which regions of the amino acid sequence of TGF-beta's 1, 2, and 3 are
substituted for each other in the mature peptide. Each of these
recombinant TGF-beta's has been purified to homogeneity using a sequence
of high pressure liquid chromatography steps. The availability of
TGF-beta 3 has permitted us to raise antibodies against this peptide
which can now be used to detect its presence in a variety of experimental
systems. Moreover, selective effects of TGF-beta 3 have been identified
in inhibition of the growth of hematopoietic cells, in migration of fetal
fibroblasts, and in inhibiting the survival of certain neuronal cells.
Using the chimeric TGF-beta constructs, we have been able to deduce that
the middle third of the TGF-beta molecule is sufficient to confer
isoform-specific biological activity. In a related aspect of this
project, we are exploring the effects of the different TGF-beta isoforms
on protection of cardiac myocytes from damage resulting from the action
of growth factors such as interleukin-I and tumor necrosis
factor-alpha.
三种不同的tgf - β亚型在哺乳动物中表达。虽然
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('A B ROBERTS', 18)}}的其他基金
STUDY OF MICE IN WHICH THE TGF BETA GENE HAS BEEN DISRUPTED
对 TGF Beta 基因被破坏的小鼠的研究
- 批准号:
2463689 - 财政年份:
- 资助金额:
-- - 项目类别:
STUDY OF MICE IN WHICH THE TGF BETA 1 GENE HAS BEEN DISRUPTED
对 TGF Beta 1 基因被破坏的小鼠的研究
- 批准号:
3752781 - 财政年份:
- 资助金额:
-- - 项目类别:
BIOLOGY AND RECEPTOR SIGNALLING OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和受体信号转导
- 批准号:
5201457 - 财政年份:
- 资助金额:
-- - 项目类别:
MECHANISM OF ACTION OF TYPE BETA TRANSFORMING GROWTH FACTOR
β型转化生长因子的作用机制
- 批准号:
4692310 - 财政年份:
- 资助金额:
-- - 项目类别:
NOVEL CHEMOPREVENTIVE AGENTS IN EXPERIMENTAL MAMMARY CARCINOGENESIS
乳腺癌实验中的新型化学预防剂
- 批准号:
6100875 - 财政年份:
- 资助金额:
-- - 项目类别:
BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
- 批准号:
3838326 - 财政年份:
- 资助金额:
-- - 项目类别:
STRUCTURE/FUNCTION AND SIGNAL TRANSDUCTION PATHWAYS OF TGF BETA
TGF Beta 的结构/功能和信号转导途径
- 批准号:
6160869 - 财政年份:
- 资助金额:
-- - 项目类别:
NOVEL CHEMOPREVENTIVE AGENTS IN EXPERIMENTAL MAMMARY CARCINOGENESIS
乳腺癌实验中的新型化学预防剂
- 批准号:
6160975 - 财政年份:
- 资助金额:
-- - 项目类别:
BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
- 批准号:
3916749 - 财政年份:
- 资助金额:
-- - 项目类别:
BIOLOGY AND MOLECULAR BIOLOGY OF TRANSFORMING GROWTH FACTOR-BETA
转化生长因子-β的生物学和分子生物学
- 批准号:
3752614 - 财政年份:
- 资助金额:
-- - 项目类别:
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