SYNTHESIS OF THYROXINE TRANSPORT PROTEINS

白细胞介素-6（IL-6）对肝细胞甲状腺激素合成的影响 激素结合蛋白的研究是通过生物合成标记人 肝母细胞瘤衍生的Hep G2细胞使用[35-S]甲硫氨酸，然后 用特异性抗血清免疫沉淀培养基， 免疫沉淀。 mRNA与特异性cDNA的狭缝印迹杂交， 也进行了。 IL-6对T4结合球蛋白产生剂量和时间依赖性抑制， 白蛋白和甲状腺素运载蛋白。 对皮质类固醇也有类似的作用- 结合球蛋白 另一方面，IL-6增加α 1-抗胰蛋白酶 和α 1-抗胰凝乳蛋白酶合成。 这些影响似乎发生在 主要是在翻译前的水平。 IL-6的活性可能是 通过与中和性抗IL 6抗血清预孵育抑制，并且可以 通过从培养基中去除细胞因子而恢复。

The effects of interleukin-6 (IL-6) on the liver synthesis of thyroid- hormone binding proteins was investigated by biosynthetic labeling of human hepatoblastoma-derived Hep G2 cells using [35-S] methionine, followed by immunoprecipitation of media with specific antisera, and fluorography of immunoprecipitates. Slot blot hybridization of mRNAs to specific cDNAs was also carried out. IL-6 produced a dose and time-dependent inhibition of T4 binding globulin, albumin and transthyretin. Similar effects were exerted on corticosteroid- binding globulin. On the other hand, IL-6 increased alpha 1-antitrypsin and alpha 1-antichymotrypsin synthesis. These effects appeared to occur mainly at a pretranslational level. The activity of IL-6 could be inhibited by preincubation with neutralizing anti-IL6 antiserum, and could be reverted by removing the cytokine from the culture medium.