MODE OF ACTION OF THYROCALCITONIN

甲状腺降钙素的作用方式

• 批准号: 3855303
• 负责人: S J MARX
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3855303
• 关键词: breast neoplasms; calcitonin; cyclic AMP; guanine nucleotide binding protein; hormone receptor; hormone regulation /control mechanism; human tissue; image processing; kidney cell; microwave radiation; neoplastic cell; nucleotide metabolism; osteocytes; protein structure function; receptor coupling; synthetic peptide; tissue /cell culture

The purpose is to study the interaction of calcitonin with its specific receptor target organs. The current investigations should provide further insight into the structure-function relationship in calcitonin. Calcitonin is a small polypeptide hormone and therefore lends itself well to studies using synthetic peptide fragments. The system is also useful for characterizing hormone receptors in kidney, bone and other tissues. Studies are in progress to characterize further the interaction of calcitonin with tissue receptors. It will also be of interest to solubilize the receptors and characterize them chemically. Calcitonin increases cAMP in MCF 7 breast cancer cells. At 300-fold lower concentration calcitonin decreases cAMP in these cells. The decrease in cAMP is prevented by preexposure of cells to agents that interfere with inhibitory guanyl regulatory proteins. Intracellular compartmentalization of cAMP accumulation after calcitonin has been imaged after microwave fixation of cells. The cAMP accumulates initially along the plasma membrane but within 1 to 3 minutes accumulates much closer to the nucleus.

目的是研究降钙素与其特异性的相互作用