MODE OF ACTION OF THYROCALCITONIN

甲状腺降钙素的作用方式

• 批准号: 3941046
• 负责人: S J MARX
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941046
• 关键词: breast neoplasms; calcitonin; chemical structure function; cyclic AMP; hormone analog; hormone regulation /control mechanism; human tissue; kidney cell; lymphatic tissue; neoplastic cell; osteocytes; peptide hormone biosynthesis; radiotracer; synthetic peptide; tissue /cell culture

The purpose is to study the interaction of calcitonin with its specific receptor target organs. The current investigations should provide further insight into the structure-function relationship in calcitonin. Calcitonin is a small polypeptide hormone and therefore lends itself well to studies using synthetic peptide fragments. The system is also useful for characterizing hormone receptors in kidney, bone and other tissues. Studies are in progress to characterize further the interaction of calcitonin with tissue receptors. It will also be of interest to solubilize the receptors and characterize them chemically. Calcitonin increases cAMP in MCF 7 breast cancer cells. At 300-fold lower concentration calcitonin decreases cAMP in these cells. The decrease in cAMP is prevented by preexposure of cells to agents that interfere with inhibitory guanyl regulatory proteins.

目的是研究降钙素与其受体的相互作用， 特异性受体靶器官。 目前的调查应 提供了进一步了解结构-功能关系， 降钙素 降钙素是一种小的多肽激素， 因此很适合于使用合成肽的研究 片段 该系统还可用于表征激素 肾脏、骨骼和其他组织中的受体。 研究正在 进一步表征降钙素与 组织受体 溶解 受体并对其进行化学表征。 降钙素升高 MCF 7乳腺癌细胞中的cAMP。 低了300倍 浓度降钙素降低这些细胞中的cAMP。 的 通过将细胞预先暴露于试剂来防止cAMP的减少 干扰抑制性鸟苷酸调节蛋白。