NATIONAL TRITIUM LABELING FACILITY

国家氚标记设施

• 批准号: 3103812
• 负责人: DAVID E WEMMER
• 金额: $ 60.15万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3103812
• 关键词: biomedical resource; chemical synthesis; radiopharmacology; radiotracer; tritium

The National Tritium Labeling Facility (NTLF) was funded on 08/01/82. During its first year the facility was built and equipped; it became fully operative in August 1983. It is now engaged in an active program of aiding biomedical researchers to obtain needed and commercially-unavailable tritium labeled compounds. For the ten month period Oct. 1983-Aug. 1984 this has averaged two labeling operations per month and has aided scientists from as far away as New York and Hawaii. The compounds labeled have included prednisone, nicotine, pheromones, gibberelic acids, endorphins, tabtoxinine- -lactam, and tuftsin. The tritium facility is now fully staffed, and because knowledge of its labeling services is growing, requests for tritiation help are also growing; the facility is expected to reach its full capacity (one tritiation per week) in its 3rd year. The core research is being rapidly pursued. During the 2nd grant year one manuscript was accepted for publication in the Journal of Physical Chemistry, and a second manuscript was submitted to that journal in September 1984. The core research summarized in these manuscripts contributes to our understanding of the processes involved in "excitation labeling" (exposure of organic compounds to a stream of T2 that has been passed through a microwave generator). The knowledge gained in this work has increased the efficiency with which the excitation procedure can be used. This research will be enhanced in the 3rd and succeeding grant years by the tritium-NMR capability that has been added. The position(s) of tritium in all labeled compounds can now be determined, and this greatly aids the mechanistic interpretations regarding the tritiation procedures that have been used. The staff of the NTLF is doing collaborative research (co-authorship of published papers) with Professor Richard E. Moore of the University of Hawaii; the work is directed towards identifying the cell-membrane receptor sites for the potent tumor promoters lyngbyatoxin and aplysiatoxin. The staff is also collaborating with Professor A.J. Kresge of the University of Toronto on hydrogen-isotope effects on NMR spectra. This kind of collaborative research will be expanded with the increasing use of the NTLF by biomedical researchers.

国家氚标记设施（NTLF）于1982年1月8日获得资助。 在第一年，该设施建成并配备了设备;它完全成为 1983年8月开始运作。 它现在正积极参与一项援助计划， 生物医学研究人员获得所需的和商业上不可用的 氚标记化合物。 1983年10月至1984年8月的10个月期间 这平均每月进行两次贴标签作业， 远至纽约和夏威夷的科学家们。 标记的化合物 包括强的松，尼古丁，信息素，赤霉酸， 内啡肽，tabtoxinine-内酰胺，和tuftsin。 氚设施现在 人员配备齐全，而且由于其标签服务的知识不断增长， 对氚化帮助的请求也在增加;该设施预计将 在第三年达到满负荷（每周一次氚化）。 核心研究正在迅速进行。 在第二个赠款年度， 手稿被接受发表在物理杂志上 化学，第二份手稿于1999年提交给该杂志。 1984年9月 这些手稿中总结的核心研究 有助于我们理解“激发”过程 标记”（将有机化合物暴露于已被 通过微波发生器）。 在这项工作中获得的知识 提高了激发过程的效率， 采用 这项研究将在第三年和随后的拨款年得到加强 通过增加的氚核磁共振能力。 的立场 现在可以确定所有标记化合物中的氚，这大大 有助于有关氚化过程的机械解释 已经被使用过了。 NTLF的工作人员正在进行合作研究（合著） 与Richard E.摩尔说， 夏威夷;这项工作是针对确定细胞膜受体 强效肿瘤促进剂灵比毒素和海兔毒素的位点。 的 工作人员还与牛津大学的A. J. Kresge教授合作， 多伦多关于氢同位素对NMR谱的影响。 这种 随着NTLF的使用越来越多，合作研究将得到扩展 被生物医学研究人员发现。