ACQUISITION OF A HIGH PERFORMANCE DOUBLE FOCUSING MASS S

研究资源司要求提供资金， 购买VG ZAB-2 VE高性能双聚焦质量块 光谱仪 该仪器的质量范围为10，000道尔顿， 加速电压为8 kV。 它将具有连续可变的 分辨率高达125，000，在20，000分辨率下透射率为2%。 建议为仪器配备焦平面电光 检测器 探测器技术的这一最新发展可以很容易地 安装和使用时无需破坏真空密封。 它包括 微通道板与线性光电二极管阵列的组合。 的 探测器可以在低分辨率（1，000）下工作，其中15%的质量范围 在高分辨率（10，000）下， 覆盖. 有了这个探测器， 质谱 最近的演示表明，这提供了一个 灵敏度提高了两个数量级， 在全扫描模式下运行。 还提出Cs+离子 枪被安装到仪器，使高灵敏度液体二次 高分子量生物分子的电离质谱可以 得到了 这种技术比快原子技术灵敏5-10倍 （氙）轰击质谱法。 因此，将有可能获得 灵敏度增加至少三个数量级， 与目前可用的双聚焦质谱仪相比 在范德比尔特。 ZAB仪器配备DEC VAX 超级微型计算机，因此将有可能实际执行 仪器的前台/后台操作， 基于DEC PDP的系统所经历的延迟， 在我们的实验室里使用。 应该可以快速处理数据 从而可以高效地使用该仪器。 长期 数据存储也可以在盒式磁带上高效地进行 胶粘带. 仪器的主要用户将来自分子 范德比尔特的毒理学中心。 将主要开展的项目 涉及蛋白质、寡核苷酸和 修饰的寡核苷酸。 一群小用户也将受益于 在项目中使用该工具， 含氧蛋白质的结构表征 重量磷脂。 预计今后， 该工具将有利于其他一些研究人员， 范德比尔特。

Funds are requested from the division of Research Resources for the purchase of a VG ZAB-2VE high performance double focusing mass spectrometer. The instrument will have a mass range of 10,000 Daltons at an accelerating voltage of 8 kV. It will have continuously variable resolution up to 125,000 with a transmission of 2% at 20,000 resolution. It is proposed to equip the instrument with a focal plane electrooptical detector. This recent development in detector technology can be readily fitted and used without having to break the vacuum seal. It consists of a microchannel plate in combination with a linear photodiode array. The detector can operate at low resolution (1,000) where 15% of the mass range is covered and at high resolution (10,000) where 4 % of the mass range is covered. With this detector it will be possible to obtain instantaneous mass spectra. Recent demonstrations have shown that this provides an increase in sensitivity of up to two orders of magnitude compared with operation in the full scanning mode. It is also proposed that a Cs+ ion gun be fitted to the instrument so that high sensitivity liquid secondary ionization mass spectra of high molecular weight biomolecules can be obtained. This technique is 5-10 times more sensitive than fast atom (xenon) bombardment mass spectrometry. Thus, it will be possible to obtain an increase in sensitivity of at least three orders of magnitude when compared with the double focusing mass spectrometers currently available at Vanderbilt. The ZAB instrument is equipped with a DEC VAX supermicrocomputer and so it will be possible to realistically carry out foreground/background operation of the instrument without the inordinate delays that are experienced with DEC PDPbased systems that are currently in use in our laboratory. It should-be possible to process data rapidly so that highly efficient use of the instrument will be possible. Long term data storage can also be carried out with great efficiency on cartridge tapes. The major users of the instrument will come form the Molecular Toxicology Center at Vanderbilt. Projects that will be carried out mainly involve the structural characterization of proteins, oligonucleotides and modified oligonucleotides. A group of minor users will also benefit in having access to the instrument in projects that range from the analysis of oxygenated proteins to structural characterization of high molecular weight phospholipids. It is anticipated that, in the future, the instrument will be of benefit to a number of other investigators at Vanderbilt.