MODULATION OF IMMUNE FUNCTIONS USING THE IMMUNOTOXIN IL2-PE40

IL-2-PE40是由IL-2组成的重组嵌合蛋白 (IL-2)和假单胞菌内毒素(PE40)，减去其细胞结合域。 这种毒素一旦内化，就会通过不可逆转的抑制作用杀死细胞 蛋白质合成。因此，表面带有IL-2受体的细胞， 它们是这种毒素的主要目标，可以有效地从 不会产生太多不良副作用。 在实验性自身免疫性葡萄膜炎(EAU)中，T细胞起主要作用。一次 激活后，这些T细胞在其细胞表面表达IL-2受体， 它可以被嵌合毒素靶向并消除。我们有 证明这种毒素能够显著降低发病率。 以及实验诱导的葡萄膜炎的严重程度。IL-2PE40也能够 减少术后角膜移植排斥反应的发生率和严重程度 全身注射毒素。

IL-2-PE40 is a recombinant chimeric protein composed of interleukin-2 (IL-2) and of Pseudomonas endotoxin (PE40), minus its cell-binding domain. This toxin, once internalized, kills cells by irreversibly inhibiting protein synthesis. Hence, cells bearing IL-2 receptors on their surface, which are prime targets for this toxin, can be effectively removed from an organism without producing too many untoward side effects. In experimental autoimmune uveitis (EAU), T cells play a major role. Once activated, these T cells express on their cell surface IL-2 receptors, which can be targeted by the chimeric toxin and eliminated. We have demonstrated that this toxin is able to reduce significantly the incidence and severity of experimentally induced uveitis. IL-2PE40 is also able to reduce the incidence and severity of corneal graft rejection following systemic administration of the toxin.