STUDIES OF HUMAN EPILEPTIC FOCUS

人类癫痫皮层的化学变化的本质是 使用体外切片技术和放射自显影进一步研究。 在体外切片中，加入去甲肾上腺素，谷氨酸，NMDA， 和生长抑素单独或联合使用， 确定癫痫病灶是否有不同的反应， 环核苷酸和磷酸肌醇水平。 的响应性 在体外条件下，癫痫组织不显著 与周围较少的癫痫组织不同 PET扫描研究 已经表明μ阿片受体的结合是轻微的 与非棘波癫痫皮质相比， 皮层 为了进一步研究这一发现， 非尖峰癫痫皮质暴露于[3-H]-DAGO，选择性μ 阿片受体配体和[3-H]-DADLE的相邻部分，μ和 δ受体配体。 研究的主要发现是， 受体位点的数量或其 在尖峰和非尖峰皮层中的亲和力。 的分布 观察到μ和δ受体是不同的。 在扣球和 非尖峰皮质，μ受体是在一个异质性的方式与 峰层在两个带之间的距离的1/3和1/2处发现， 脑膜和白色物质。 在相同的组织中，δ受体 均匀分布，但确实具有最高数量的梯度 位于皮层最外层的感受器。 的数量 TCP受体在尖峰与nonspiking皮层也进行了研究 使用[3-H]-TCP。 结果是，没有受体数量或 当比较加标和非加标组织时，亲和力变化。 TCP受体在两种组织中的分布相似， 最外层受体数量最多的梯度 皮质层。

The nature of the chemical alterations in the human epileptic cortex were investigated further using in vitro slice techniques and autoradiography. The in vitro slices, and the addition of norepinephrine, glutamate, NMDA, and somatostatin were used individually or in combination in order to determine whether the epileptic focus had a different responsiveness at the level of cyclic nucleotides and inositol phosphate. The responsiveness of the epileptic tissue under in vitro conditions was not significantly different from the surrounding less epileptic tissue. PET scan studies have indicated that the binding of the mu opiod receptor was slightly elevated in the spiking epileptic cortex when compared to the nonspiking cortex. To investigate this finding further, sections of the spiking and nonspiking epileptic cortex were exposed to [3-H]-DAGO, a selective mu opiate receptor ligand, and adjacent sections to [3-H)-DADLE, a mu and delta receptor ligand. The major findings of the study are that there are no significant differences for the number of receptor sites or their affinity in the spiking versus nonspiking cortex. The distribution of the mu and delta receptors was observed to be different. In the spiking and nonspiking cortex, the mu receptors were in a heterogeneous manner with the peak layers found in two bands at 1/3 and 1/2 the distance between the meninges and the white matter. In the same tissues, the delta receptor was homogeneously distributed but did have a gradient with the highest number of receptors located in the outermost layer of the cortex. The number of TCP receptors in the spiking versus nonspiking cortex was also investigated using [3-H]-TCP. The findings were that there were no receptor number or affinity changes when the spiking and nonspiking tissues were compared. The distribution of TCP receptors was similar in the two tissues and had a gradient with the highest number of receptors localized in the outermost layers of the cortex.