TRANSPLANT OF GABAERGIC NEURONS INTO THE SUBSTANTIA NIGRA OF KINDLED RATS

将伽巴能神经元移植到点燃大鼠的黑质中

• 批准号: 3922619
• 负责人: S NADI
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3922619
• 关键词: GABA receptor; antibody; cerebellum; convulsions; early embryonic stage; embryo /fetus; epilepsy; laboratory rat; nervous system transplantation; neurons; stainings; substantia nigra

This project will investigate the effect of transplanting neuronal suspensions from the cerebellum of 16 day old rat embryos into the substantia nigra of adult kindled rats. The hypothesis is that the substantia nigra is involved in the control of spread of seizures and the addition of gamma amino butyric acid (GABA)ergic neurons to the nigra will prevent the seizures. GABA ergic neurons are obtained by gentle trypsinization of the cerebellum of 16 days old embryos. These cells are then transplanted into the substania nigra using a Harvard pump. The origin of the cells will be determined by staining with the glutamate decarboxylase (GAD) antibody as well as cerebellum specific antibodies. Our most recent results show at a light microscopic level a fine network of cells at the area of transplant in the nigra. These cells are elongated in shape with extensive processes. They have failed to stain with GAD antibody, but other GABA ergic neurons in the area have not stained either. We are currently investigating the reasons behind this. Preliminary work has shown that kindling disappears for 2 weeks after transplant but then returns. The reasons for these findings are being investigated. The substantia nigra has been shown to be a controlling point of seizure spread by numerous investigators. The current study will attempt to demonstrate whether transplants of GABA ergic neurons from rat embryo cerebellum will block the spread of seizures.

本课题将探讨神经元移植对大鼠脑缺血再灌注损伤的影响 将16日龄大鼠胚胎小脑的悬浮液注入 点燃成年大鼠黑质。 假设是， 黑质参与控制癫痫的蔓延 以及添加γ-氨基丁酸（GABA）能神经元 就能防止癫痫发作 GABA能神经元通过温和的胰蛋白酶消化获得。 16日龄胚胎小脑。 然后这些细胞 用哈佛泵移植到黑质 的 细胞的来源将通过用 谷氨酸脱羧酶（GAD）抗体以及小脑 特异性抗体 我们最近的结果显示，在光显微镜水平上， 移植区域的细胞网络。 这些 细胞呈细长形，突起广泛。 他们有 GAD抗体未能染色，但其他GABA能神经元， 该地区也没有染色。 我们目前正在调查 这背后的原因。 初步研究表明， 移植后两周消失，然后又回来了。 的 目前正在调查这些发现的原因。 黑质已被证明是一个控制点， 许多调查人员传播了缉获量。 本研究将 试图证明GABA能神经元的移植 会阻止癫痫的蔓延。