PLASMA AND CSF LEVELS OF NEUROTRANSMITTERS IN EPILEPTICS AND EKG COLLELATION

血液和脑脊液(CSF)样本将以 癫痫患者癫痫发作前、后。样品将会 在等份中冷冻储存，并通过以下方法检测多肽 用荧光法测定氨基酸的放射免疫测定法 儿茶酚胺类化合物的高效液相色谱分析 连接到一个电化学探测器。这些发现将是 与任何脑电(EEG)或 大鼠癫痫发作时的心电图改变 以期对癫痫猝死综合征有一些认识 可能会得到。 生长抑素、神经降压素、P物质、β-内毒素水平 内啡肽、脑啡肽、蛙皮素、神经肽Y、催乳素、促肾上腺皮质激素、 测定促肾上腺皮质激素释放因子、促胆囊素 未发现有显著差异。23个氨基酸的水平 还测定了酸，但没有明显的变化 观察感染前后脑脊液中的变化。 在脑脊液中检测到哇巴因样因子。 术后脑脊液中这一因子升高。此外，一个 发现一条相对分子质量为10万的蛋白质条带 在死后早期被磷酸化，并失去了它的 死后晚期的磷酸化状态。 去甲肾上腺素、高香草酸(HVA)、5-甲氧基苯丙氨酸(5- 脑脊液中的羟基吲哚乙酸(5HIAA)均升高。

Blood and cerebrospinal fluid (CSF) samples will be obtained form patients with epilepsy before and after seizure. The sample will be stored frozen in aliquots, and assayed for peptides by radioimmunoassay, for amino acids by fluorescence, and for catecholamines by high performance liquid chromatography (HPLC) linked to an electrochemical detector. These findings will be correlated to any electroencephalographic (EEG) or electrocardiographic (EKG) changes which occur during seizures in order that some insight int the sudden death syndrome in epilepsy might be obtained. The level of peptides somatosatin, neurotensin, substance P, Beta endrophin, enkephalin, bombesin, neuropeptide Y, prolactin, ACTH, corticotropin releasing factor, cholecystokinin (CCK) were studied and no significant differences were found. The level of 23 amino acids were also determine but no significant alterations were observed in pre- and postictal CSF. The presence of an ouabain like factor was detected in the CSF. This factor was elevated in the CSF postictally. Furthermore a band of protein of molecular weight of 100,000 was found to be phosphorylated in the early postictal phase and lose its phosphorylated state in the late postictal phase. Postictal levels of norepinephrine, homovanillic acid (HVA), 5- hydroxyindole acetic acid (5HIAA) were all elevated in the CSF.