MOUSE STRAIN SUSCEPTIBILITY TO BACILLUS PILIFORMIS

小鼠品系对毛状芽孢杆菌的敏感性

• 批准号: 3916165
• 负责人: K S WAGGIE
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916165
• 关键词: Bacillus; bactericidal immunity; communicable disease transmission; disease /disorder model; genetic strain; gram negative bacteria; immunodeficiency; laboratory mouse; model design /development; virulence

The objective of this project was to find a mouse strain that is susceptible to B. piliformis infection via the oral (natural) route. A susceptible strain would aid in pathogenesis studies and might act as sentinel animals to detect naturally occurring infections in rodent colonies. Mice with various immunodeficiencies were orally inoculated with B. piliformis spores. Mice were killed 5 days post-inoculation and examined for gross lesions. Tissues were also collected for histopathology. Mouse strains utilized were as follows: CBA/N, P/N, C3H/HeJ, C3H/HeN (C3H), C3H-nu, C3H-bg, C3H-xid, C3H-bg,nu; C3H-bg,xid; C3H-nu,xid. No significant gross lesions were present in any of the inoculated mice. Cecoco-litis was microscopically evident in C3H-bg,nu mice, however, no B. piliformis-like bacteria were observed in silver stained sections. Further studies need to be done to determine if C3H-bg,nu mice are susceptible to B. piliformis infection and, if so, if they would be suitable sentinel animal.

这个项目的目标是找到一种小鼠品系， 对B敏感。通过口腔（自然）途径感染毛状菌。 一 敏感菌株将有助于致病机理研究， 哨兵动物检测啮齿动物中自然发生的感染 殖民地 用B经口接种具有各种免疫缺陷的小鼠。 毛状孢子 接种后5天处死小鼠并检查 肉眼可见的损伤 还采集组织用于组织病理学检查。 鼠标 使用的菌株如下：CBA/N，P/N，C3 H/HeJ，C3 H/HeN（C3 H）， C3H-nu，C3H-bg，C3H-xid，C3H-bg，nu; C3H-bg，xid; C3H-nu，xid。 在任何接种小鼠中均不存在显著的肉眼病变。 C3 H-bg，nu小鼠显微镜下可见盲肠炎，但无B。 在银染色切片中观察到毛状菌样细菌。 进一步 需要进行研究以确定C3 H-bg，nu小鼠是否对 B。如果是的话，他们是否会成为合适的哨兵 动物