MOUSE STRAIN SUSCEPTIBILITY TO BACILLUS PILIFORMIS

小鼠品系对毛状芽孢杆菌的敏感性

• 批准号: 3937279
• 负责人: K S WAGGIE
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3937279
• 关键词: animal colony; bactericidal immunity; communicable disease transmission; genetic strain; gram negative bacteria; laboratory rat; virulence

The objective of this project was to find a mouse strain that is susceptible to B. piliformis infection via the oral (natural) route. A susceptible strain would aid in pathogenesis studies and might act as sentinel animals to detect naturally occurring infections in rodent colonies. Mice with various immunodeficiencies were orally inoculated with B. piliformis spores. Mice were killed 5 days post-inoculation and examined for gross lesions. Tissues were also collected for histopathology. Mouse strains utilized were as follows: CBA/N, P/N, C3H/HeJ, C3H/HeN (C3H), C3H-nu, C3H-bg, C3H-xid, C3H-bg,nu; C3H-bg,xid; C3H-nu,xid. No significant gross lesions were present in any of the inoculated mice. Cecocolitis was microscopically evident in C3H-bg,nu mice, however, no B. piliformis like bacteria were observed in silver stained sections. Further studies need to be done to determine if C3H-bg,nu mice are susceptible to B. piliformis infection and, if so, if they would be a suitable sentinel animal.

这个项目的目标是找到一种小鼠品系， 对B敏感。 通过口腔感染（自然） 路线 敏感菌株将有助于致病机理研究， 可以作为哨兵动物来检测自然发生的 啮齿类动物的感染。 具有各种免疫缺陷的小鼠经口接种 B。毛状孢子 接种后5天处死小鼠， 检查是否有肉眼病变。 还收集了组织， 组织病理学 使用的小鼠品系如下：CBA/N， P/N、C3H/HeJ、C3H/HeN（C3H）、C3H-nu、C3H-bg、C3H-xid、C3H-bg、nu; C3H-bg，xid; C3H-nu，xid. 在所有接种的小鼠中均不存在显著的肉眼病变。 小鼠 在C3 H-bg，nu小鼠中显微镜下可见盲肠结肠炎， 然而，没有B。在银中观察到纤毛状细菌 染色切片。 还需要进一步的研究来确定， C3 H-bg，nu小鼠对B易感。感染，如果 所以它们是否适合做哨兵动物