SIGNAL GENERATION AND SECRETION OF MEDIATORS IN RAT BASOPHIL LEUKEMIC CELLS

大鼠嗜碱性粒细胞白血病细胞中信号的产生和介质的分泌

• 批准号: 3920017
• 负责人: T N LO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3920017
• 关键词: adenosine; arachidonate; basophils; calcium metabolism; cholera toxin; exocytosis; histamine release; ionophores; laboratory rat; neoplastic cell culture for noncancer research; nucleoside analog; phorbols; phosphatidylinositols; phosphodiesterase inhibitors; protein kinase C; secretion; tissue /cell culture

Ca2+-Ionophore-induced secretion of histamine from rat basophil leukemic (RBL-2H3) cell correlated with the hydrolysis of inositol phospholipids but not with the increase in cytosol (Ca(2+)). However, external (Ca(2+)) was obligatory for secretion (J. Biol. Chem. 262:414,1987). The same pattern was observed for release of arachidonic acid. Similarities were noted in release of histamine and arachidonic acid in antigen-stimulated cells. The release of both were enhanced by treatment with cholera toxin and by the presence of andenosine analogs and were inhibited by the phosphodiesterase inhibitor IBMX in either intact or permeabilized cells. Although their release may depend on the generation of the same signals at the level of the membrane the subsequent mechanisms for release probably diverge: the release of arachidonic acid was 4 to 5 times greater with optimal concentrations of A23187 than with optimal concentrations of antigen whereas the reverse was true for release of histamine. Also inhibitors of protein kinase C or depletion of protein kinase (by prolonged exposure of cells to phorbol ester) resulted in reduced secretion of histamine but enhanced release of arachidonic acid upon stimulation of cells with antigen.

Ca2+-离子载体诱导大鼠嗜碱性粒细胞分泌组胺 白血病（RBL-2H3）细胞与肌醇水解相关 磷脂但不随细胞质 (Ca(2+)) 的增加而增加。 然而，外部（Ca(2+)）对于分泌是必需的（J. Biol. 化学。 262：414，1987）。 观察到相同的模式释放 花生四烯酸。 在组胺的释放方面发现了相似之处 和抗原刺激细胞中的花生四烯酸。 的释放 两者均通过霍乱毒素治疗和 腺苷类似物的存在并被抑制 完整或透化的磷酸二酯酶抑制剂 IBMX 细胞。 尽管它们的发布可能取决于生成的 膜水平上的相同信号以及后续机制 释放可能有所不同：花生四烯酸的释放是 A23187 的最佳浓度比其高 4 至 5 倍 具有最佳抗原浓度，而反之亦然 用于释放组胺。 还有蛋白激酶 C 抑制剂或 蛋白激酶的消耗（通过使细胞长时间暴露于 佛波酯）导致组胺分泌减少，但 刺激细胞后花生四烯酸的释放增强 抗原。