ATP DEPENDENCY OF SIGNAL GENERATION AND SECRETION IN RAT BASOPHIL LEUKEMIC CELLS

大鼠白血病嗜碱性粒细胞(2H3)细胞分泌组胺的实验研究 通过钙离子载体或通过血浆聚集 Ig E的膜受体。离子载体，A23187，在浓度(小于 超过100 NM)远低于刺激分泌物引起的大量 高浓度(200-1000 nm)刺激的(Ca~(2+))i升高 膜上放射性标记的肌醇磷脂的水解性。这个 水解度依赖于离子载体的浓度和 细胞外钙与分泌反应呈正相关。结果是 指的是二酰甘油的生成，而不是肌醇磷酸 作为A23187行动中的一个关键因素。当分泌性反应 被绘制为肌醇磷脂的百分比水解度的函数， 在佛波酯TPA存在的情况下，曲线左移。 和二酰甘油一样，它也是蛋白激酶C的激活剂。 矛盾的是，(钙)i的增加对抗原和 A23187高度依赖于细胞内的ATP水平。

Rat leukemic basophil (2H3) cell line was stimulated to secrete histamine either with calcium-specific ionophores or by aggregation of plasma membrane receptors for IgE. The ionophore, A23187, at concentrations (less than 100 nM) well below those stimulating secretion elicited large increases in (Ca2+)i and at higher concentrations (200-1000nM) stimulated hydrolysis of membrane radiolabeled inositol phospholipids as well. The hydrolysis was dependent on the concentration of ionophore and presence of external Ca2+ and was correlated with the secretory response. The results pointed to generation of diacylglycerol rather than of inositol phosphates as a critical factor in the action of A23187. When secretory responses were plotted as a function of percent hydrolysis of inositol phospholipid, the curve was shifted leftwards in the presence of the phorbol ester, TPA, which, like diacylglycerol, is an activator of protein kinase C. Paradoxically, the increases in (Ca2+)i in response to both antigen and A23187 were highly dependent on intracellular ATP levels.