ATP DEPENDENCY OF SIGNAL GENERATION AND SECRETION IN RAT BASOPHIL LEUKEMIC CELLS

刺激大鼠白血病嗜碱性粒细胞（2 H3）分泌组胺 用钙特异性离子载体或通过血浆聚集 IgE的膜受体。 离子载体A23187在浓度（小于 低于100 nM），远远低于刺激分泌的水平， 增加（Ca 2+）i和在较高浓度（200- 1000 nM）刺激 膜放射性标记的肌醇磷脂的水解。 的 水解依赖于离子载体的浓度和 细胞外Ca ~（2+）与分泌反应相关。 结果 指出生成甘油二酯而不是肌醇磷酸 作为A23187作用的关键因素。 当分泌反应 作为肌醇磷脂水解百分比的函数作图， 在佛波醇酯，TPA， 其与二酰基甘油一样是蛋白激酶C的激活剂。 特异性地，（Ca 2+）i响应于抗原和 A23187高度依赖于细胞内ATP水平。

Rat leukemic basophil (2H3) cell line was stimulated to secrete histamine either with calcium-specific ionophores or by aggregation of plasma membrane receptors for IgE. The ionophore, A23187, at concentrations (less than 100 nM) well below those stimulating secretion elicited large increases in (Ca2+)i and at higher concentrations (200-1000nM) stimulated hydrolysis of membrane radiolabeled inositol phospholipids as well. The hydrolysis was dependent on the concentration of ionophore and presence of external Ca2+ and was correlated with the secretory response. The results pointed to generation of diacylglycerol rather than of inositol phosphates as a critical factor in the action of A23187. When secretory responses were plotted as a function of percent hydrolysis of inositol phospholipid, the curve was shifted leftwards in the presence of the phorbol ester, TPA, which, like diacylglycerol, is an activator of protein kinase C. Paradoxically, the increases in (Ca2+)i in response to both antigen and A23187 were highly dependent on intracellular ATP levels.