SIGNAL GENERATION AND SECRETION OF MEDIATORS IN RAT BASOPHIL LEUKEMIC CELLS

大鼠嗜碱性粒细胞白血病细胞中信号的产生和介质的分泌

• 批准号: 3942801
• 负责人: T N LO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942801
• 关键词: arachidonate; basophils; calcium metabolism; enzyme induction /repression; histamine release; ionophores; neoplastic cell culture for noncancer research; phosphatidylinositols; protein kinase C; tissue /cell culture

Ionophore-induced secretion of histamine from rat basophil leukemic (RBL-2H3) cells correlated with the hydrolysis of inositol phospholipids but not with the increase in cytosol Ca(2+) ((Ca(2+))) although increased (Ca(2+))) was obligatory for secretion (J. Boil Chem. 262:414 1987). The same pattern was observed for release for arachidonic acid. Moreover, the release of both histamine and arachidonic acid was enhance by activators of protein kinase C and in this circumstance the release was correlated with the increase in (Ca(2+))). The extent of release of histamine and arachidonic acid to ionophore alone, therefore, may be limited by the extent of activation of protein kinase C rather than by the extent of increase in (Ca(2+))). The subsequent mechanisms of release may well differ: the release of arachidonic acid was 4 to 5 times greater with optimal concentrations of A23187 than with optimal concentrations of antigen in the absence or presence of activators of protein kinase C. This was not true for release of histamine for which both ionophore and an activator of protein kinase C were required to match the release induced by optimal concentrations of antigen alone.

离子载体诱导大鼠嗜碱性粒细胞分泌组胺 白血病（RBL-2H3）细胞与水解相关 肌醇磷脂但不随胞质 Ca(2+) 增加 ((Ca(2+))) 虽然增加 (Ca(2+))) 是分泌所必需的 (J. Boil Chem. 262:414 1987)。 观察到相同的模式 释放花生四烯酸。 此外，两者的发布 组胺和花生四烯酸通过激活剂增强 蛋白激酶 C，在这种情况下，释放是 与(Ca(2+)))的增加相关。 释放程度 因此，组胺和花生四烯酸单独作用于离子载体，可能 受蛋白激酶 C 激活程度的限制 比(Ca(2+)))增加的程度。 随后的 释放机制可能很不同：释放 最佳状态下花生四烯酸含量增加 4 至 5 倍 A23187 的浓度比最佳浓度 存在或不存在蛋白激酶激活剂时的抗原 C. 对于组胺的释放而言，情况并非如此，因为组胺的释放 需要离子载体和蛋白激酶 C 激活剂 与最佳抗原浓度诱导的释放相匹配 独自的。