THE MECHANISM OF CARRAGEENAN INDUCED INFLAMMATION IN RAT

卡拉胶引起大鼠炎症的机制

• 批准号: 4694489
• 负责人: T N LO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694489
• 关键词: antiinflammatory agents; antiserum; arachidonate; blood proteins; carrageenan; complement; histamine release; indomethacin; inflammation; neutrophil; phagocytosis; pharmacology; prostaglandins; serotonin; vascular endothelium permeability

Cells isolated from the pleural cavity of rat consisted mainly of macrophages, mast cells, eosinophils, lymphocytes and mesothelial cells. Isolated pleural cells labeled with [14C]-arachidonic acid released substantial amounts of radiolabel upon exposure to pharmacologically relevant concentration of carrangeenan (l-100 ug/ml). The time course of release was also compatible with the in vivo inflammatory action of carrageenan. An inhibitor of phospholipase A2 (i.e., p-bromophenacyl bromide) but not of arachidonate cyclooxygenase (i.e., indomethacin) inhibited the release in a dose-dependent manner. Analysis (by HPLC and radioimmunoassay) of the released products indicated that more than 90% of the radiolabel was arachidonic acid. Carrageenan also caused significant release of lactate dehydrogenase (LDH) from pleural cells. Release of LDH generally paralleled release of radiolabel although release of radiolabel preceded that of LDH. That the two events did not bear a cause-effect relationship, however, was demonstrated by the failure of p-bromophenacyl bromide to block release of LDH.

从大鼠胸膜腔分离的细胞主要由以下组成： 巨噬细胞、肥大细胞、嗜酸性粒细胞、淋巴细胞和间皮细胞。 释放[14 C]-花生四烯酸标记的分离胸膜细胞 暴露于放射性物质后， 卡拉胶的相关浓度（1 -100 μ g/ml）。 的时间过程 释放也与体内炎症作用相容， 卡拉胶。 磷脂酶A2的抑制剂（即，对溴苯甲酰甲基 溴化物）而不是花生四烯酸环加氧酶（即，吲哚美辛） 以剂量依赖性方式抑制释放。 分析（通过HPLC和 放射免疫测定法）表明，超过90%的释放产物， 放射性标记是花生四烯酸。 角叉菜胶也引起了严重的 胸膜细胞释放乳酸脱氢酶（LDH）。 LDH释放量 尽管放射性标记的释放 在LDH之前。 这两件事没有因果关系 然而，对溴苯甲酰甲基的失败证明了这种关系 溴化物以阻断LDH的释放。