HEWLETT PACKARD GAS CHROMATOGRAPH/MASS SPECTROMETER MODE

惠普气相色谱仪/质谱仪模式

• 批准号: 3519919
• 负责人: William H Dietz
• 金额: $ 15.7万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-11 至 1989-04-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519919
• 关键词: aminoacid metabolism; biomedical equipment purchase; computers; gas chromatography mass spectrometry

This proposal requests funds to purchase a Hewlett Packard Gas Chromatograph/Mass Spectrometer Model 5988A to be used by a large group of clinical investigators at the Tufts University School of Medicine. The users group includes a wide range of investigators in the Departments of Medicine and Pediatrics, as well as clinical investigators based at the Tufts Human Nutrition Research Center for the Study of Aging. The needs of the investigators who form the users group are primarily for repetitive analyses of amino acids. 13C-leucine is used to study protein metabolism under the conditions of hypocaloric dietary therapy, exercise, and Crohn's disease. 6,6,d2-glucose is used to study the interrelationships of glucose and protein under hypocaloric dietary connections, glucose metabolism during a euglycemic clamp, and glucose recycling in normal and diabetic humans. A novel proposal utilizes d2-folate to examine folate turnover in aging humans. Incorporation of d3- leucine into apolipoproteins is used as a tracer and to assess apolipoprotien turnover. Although several of the major users have established collaborations with GC-MS Centers elsewhere in the Boston area, several developments already limit the availability and utilization of these instruments. Heavy competition with other investigators using stable isotope technology already exists: long delays in sample analysis are common. These hamper the analysis of the pilot studies necessary to the design and conduct of more intrusive investigations. Second, the delay of results of earlier studies mean that additional cannot be initiated. Both of these factors limit the expansion of research projects already receiving PHS support. Finally, because these instruments are not committed to the support of Tufts investigators, analysis of our specimens is accorded a low priority, and makes our future of these methods tenuous. Acquisition of a GC-MS at our institution would relieve the analytic pressure we now face, and assure the completion of projects already underway. Furthermore, this instrument would improve our potential for related research in the future, and expose new clinical investigators to stable isotope technology.

该提案要求资金购买惠普 色谱仪/质谱仪型5988A型号 塔夫茨大学学校的大量临床研究人员 医学。 用户组包括广泛的 医学和儿科部门的调查员 以及基于人类营养的临床研究人员 衰老研究中心。 组成用户组的调查人员的需求是 主要用于对氨基酸的重复分析。 13C亮氨酸是 用于研究蛋白质代谢的条件 低温饮食疗法，运动和克罗恩病。 6,6，D2-葡萄糖用于研究葡萄糖的相互关系 和蛋白质在低温饮食连接下，葡萄糖 在葡萄糖夹具期间的代谢，葡萄糖回收在 正常和糖尿病人。 一个新颖的建议利用D2叶酸 检查衰老人类的叶酸周转率。 掺入D3- 亮氨酸进入载脂蛋白被用作示踪剂并评估 Apolipoprotien流失率。 尽管几个主要用户有 与GC-MS中心建立了合作 波士顿地区，几个发展已经限制了可用性 以及这些乐器的利用。 与之激烈的竞争 其他研究人员已经使用稳定的同位素技术 存在：样本分析中的长时间延迟很常见。 这些障碍 对设计必不可少的试点研究的分析 进行更具侵入性的调查。 第二，延迟 早期研究的结果意味着无法启动其他。 这两个因素都限制了研究项目的扩展 已经收到PHS支持。 最后，因为这些 乐器不致力于簇 研究人员，对我们的标本的分析是一个低优先级， 并使我们的这些方法的未来脆弱。 在我们机构中获取GC-MS将减轻 我们现在面临的分析压力，并确保完成 项目正在进行中。 此外，这个乐器将 提高我们将来的相关研究潜力，并 将新的临床研究人员暴露于稳定的同位素技术中。