LTQ Linear Ion Trap Mass Spectrometer System

LTQ 线性离子阱质谱仪系统

• 批准号: 6879828
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 49.72万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879828
• 关键词: biomedical equipment purchase; computers; high performance liquid chromatography; mass spectrometry; phosphopeptides; protein quantitation /detection

DESCRIPTION (provided by applicant): Funds are requested to purchase a Thermo Finnigan LTQ linear ion trap mass spectrometer, accompanying micro HPLC system and computer cluster for the Institutional Mass Spectrometry Laboratory (MSL) of the University of Texas Health Science Center at San Antonio, under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. At the present time, capillary HPLC-ESI/ MS/MS analyses are conducted on a Thermo Finnigan LCQ-Classic that was installed in 1997, and MALDI-TOF mass spectra are acquired on an Applied Biosystems Voyager-DE STR. These instruments have been extraordinarily powerful tools for identification and characterization of proteins and peptides for a large number of investigators. The 2003 user group consisted of 36 individual faculty members from 14 departments at UTHSCSA in addition to a number of researchers from other institutions in Texas and elsewhere in the U.S. Several technological limitations of the LCQ-Classic along with concerns about its operating lifetime prompted efforts to seek funding for a new instrument for the MSL. Regarding the performance of the instrument, improvements in detection limits, scan speed and sample through-put would benefit a large proportion of the user group. Advantages of the LTQ related to routine maintenance would enhance efficiency of day-to-day usage. Dr. Weintraub evaluated the LTQ during a trip to San Jose in February 2004 at which time she analyzed representative samples prepared in her laboratory. The results clearly demonstrated the significant benefits to the user group of the MSL that will result from acquisition of an LTQ in the following areas: (1) identification of low levels of protein in a gel; (2) identification of a low level of protein in a complex mixture in a gel; and (3) detection of a phosphopeptide at low stoichiometry. Project descriptions for 9 major users, 4 minor users and 3 future users are presented.

描述(由申请人提供)：申请资金为德克萨斯大学圣安东尼奥分校健康科学中心的机构质谱室(MSL)购买一台Thermo Finnigan LTQ线性离子陷阱质谱仪，附带的微型高效液相色谱仪系统和计算机集群，指导苏珊·温特劳布博士，她在生物医学质谱学方面有30多年的经验。目前，在1997年安装的Thermo Finnigan LCQ-Classic上进行了毛细管HPLC-ESI/MS/MS分析，在应用生物系统Voyager-DE STR上获得了MALDI-TOF质谱图。对于大量的研究人员来说，这些仪器已经成为鉴定和表征蛋白质和多肽的非常强大的工具。2003年的用户组包括来自UTHSCSA 14个系的36名个别教员，以及来自德克萨斯州和美国其他地方的其他机构的一些研究人员。LCQ-Classic的几个技术限制以及对其操作寿命的担忧促使人们努力为MSL寻求新仪器的资金。关于仪器的性能，在检测限度、扫描速度和样品产量方面的改进将使很大一部分用户群体受益。LTQ在日常维护方面的优势将提高日常使用的效率。温特劳布博士在2004年2月的一次圣何塞之旅中对LTQ进行了评估，当时她分析了在她的实验室准备的代表性样本。结果清楚地表明，获得LTQ将在以下方面对MSL的用户组产生重大好处：(1)识别凝胶中的低水平蛋白质；(2)识别凝胶中复杂混合物中的低水平蛋白质；以及(3)在低化学计量比下检测到磷酸肽。给出了9个大用户、4个小用户和3个未来用户的项目说明。

项目成果

Vaccine-mediated immune responses to experimental pulmonary Cryptococcus gattii infection in mice.

• DOI: 10.1371/journal.pone.0104316
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Chaturvedi AK;Hameed RS;Wozniak KL;Hole CR;Leopold Wager CM;Weintraub ST;Lopez-Ribot JL;Wormley FL Jr
• 通讯作者: Wormley FL Jr