LTQ Linear Ion Trap Mass Spectrometer System

LTQ 线性离子阱质谱仪系统

• 批准号: 6879828
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 49.72万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879828
• 关键词: biomedical equipment purchase; computers; high performance liquid chromatography; mass spectrometry; phosphopeptides; protein quantitation /detection

DESCRIPTION (provided by applicant): Funds are requested to purchase a Thermo Finnigan LTQ linear ion trap mass spectrometer, accompanying micro HPLC system and computer cluster for the Institutional Mass Spectrometry Laboratory (MSL) of the University of Texas Health Science Center at San Antonio, under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. At the present time, capillary HPLC-ESI/ MS/MS analyses are conducted on a Thermo Finnigan LCQ-Classic that was installed in 1997, and MALDI-TOF mass spectra are acquired on an Applied Biosystems Voyager-DE STR. These instruments have been extraordinarily powerful tools for identification and characterization of proteins and peptides for a large number of investigators. The 2003 user group consisted of 36 individual faculty members from 14 departments at UTHSCSA in addition to a number of researchers from other institutions in Texas and elsewhere in the U.S. Several technological limitations of the LCQ-Classic along with concerns about its operating lifetime prompted efforts to seek funding for a new instrument for the MSL. Regarding the performance of the instrument, improvements in detection limits, scan speed and sample through-put would benefit a large proportion of the user group. Advantages of the LTQ related to routine maintenance would enhance efficiency of day-to-day usage. Dr. Weintraub evaluated the LTQ during a trip to San Jose in February 2004 at which time she analyzed representative samples prepared in her laboratory. The results clearly demonstrated the significant benefits to the user group of the MSL that will result from acquisition of an LTQ in the following areas: (1) identification of low levels of protein in a gel; (2) identification of a low level of protein in a complex mixture in a gel; and (3) detection of a phosphopeptide at low stoichiometry. Project descriptions for 9 major users, 4 minor users and 3 future users are presented.

描述（由申请人提供）：要求资金购买热芬尼甘（LTQ LTQ线性离子陷阱质谱仪），伴随的Micro HPLC系统和计算机群集，用于在San Antonio的德克萨斯大学健康科学中心的机构质谱实验室（MSL），在Susan Weintraub博士的指导下，在San Antonio的指导下，拥有30年以上的经验。目前，毛细管HPLC-ESI/ MS/ MS分析是在1997年安装的Thermo Finnigan LCQ-Classic上进行的，并在Applied Biosystems Voyager-De str上获得了MALDI-TOF质谱。这些仪器是识别和表征大量研究人员的蛋白质和肽的极大工具。 2003年的用户组由来自UTHSCSA的14个部门的36位个人教职员工组成，除了来自德克萨斯州和美国其他地方的许多研究人员外，LCQ-Classic的一些技术局限性以及对其运营终生的担忧促使其努力促进了为MSL寻求新工具的努力。关于仪器的性能，提高检测极限，扫描速度和样本的贯穿速度将使大部分用户组受益。与常规维护有关的LTQ的优势将提高日常使用的效率。 Weintraub博士在2004年2月前往圣何塞的旅行中评估了LTQ，然后她分析了在实验室准备的代表样本。结果清楚地证明了MSL的用户群体在以下区域中获取LTQ所产生的显着好处：（1）凝胶中低水平的蛋白质识别； （2）凝胶中复杂混合物中低水平的蛋白质鉴定； （3）在低化学计量时检测磷酸肽。介绍了9个主要用户，4个未来用户和3个未来用户的项目描述。

项目成果

Vaccine-mediated immune responses to experimental pulmonary Cryptococcus gattii infection in mice.

• DOI: 10.1371/journal.pone.0104316
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Chaturvedi AK;Hameed RS;Wozniak KL;Hole CR;Leopold Wager CM;Weintraub ST;Lopez-Ribot JL;Wormley FL Jr
• 通讯作者: Wormley FL Jr