MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

已经开发了用于分离的分析方法， 元素（主要是金属）的定量和表征 以及它们的络合物（配位化合物）， 微量生物样本 体内和体外研究 旨在产生生物学相关结果， 分离和定量低浓度金属的能力 浓度的获得以及能力， 生物环境及其组分的浓度 数量上。 了解研究药物的作用机制 了解金属与金属之间的相互作用 与蛋白质和其他成分的复合物。 不幸 文献中报道的许多研究都是在 由于缺乏分析方法， 必要的敏感性。 在铂类药物的情况下， 是相对缓慢的动力学中的另一个复杂因素， 一些反应;许多已发表的研究假设， 金属络合物物种及其相互转化。

Analytical methods have been developed for separation, quantitation and characterization of elements (principally metals) and their complex species (coordination compounds) present in biological samples at trace levels. In vivo and in vitro studies designed to produce biologically relevant results require the ability to separate and quantitate the metals in the low concentrations obtaining as well as the ability to relate their concentrations to the biological milieux and their components quantitatively. To understand the mechanisms of action of the drugs under study it is important to understand the interactions of the metal complexes with proteins and other constituents. Unfortunately many studies reported in the literature have been conducted at much higher concentrations for want of analytical methods with the requisite sensitivity. In the case of the platinum drugs there is a further complicating factor in the relatively slow kinetics of some of the reactions; many published studies assume that the metal complex species and their interconversions are.