MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

生物环境中痕量金属和复合物的测量

• 批准号: 3959953
• 负责人: A F LEROY
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3959953
• 关键词: analytical method; atomic absorption spectrometry; biotransformation; chromatography; cis platinum compound; clinical chemistry; high performance liquid chromatography; human tissue; metal complex; pharmacokinetics; plasma; platinum; trace elements; vanadium

Analytical methods have been developed for separation, quantitation and characterization of elements (principally metals) and their complex species (coordination compounds) present in biological samples at trace levels. In vivo and in vitro studies designed to produce biologically relevant results require the ability to separate and quantitate the metals in the low concentrations obtaining as well as the ability to relate their concentrations to the biological milieux and their components quantitatively. To understand the mechanisms of action of the drugs under study it is important to understand the interactions of the metal complexes with proteins and other constituents. Unfortunately many studies reported in the literature have been conducted at much higher concentrations for want of analytical methods with the requisite sensitivity. In the case of the platinum drugs there is a further complicating factor in the relatively slow kinetics of some of the reactions; many published studies assume that the metal complex species and their interconversions are.

分离的分析方法已经开发出来，