MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

已开发出用于分离的分析方法， 元素（主要是金属）的定量和表征 及其复杂种类（配位化合物）存在于 痕量水平的生物样品。 体内和体外研究 旨在产生生物学相关的结果需要 能够在低浓度下分离和定量金属 获得的浓度以及将它们联系起来的能力 生物环境及其成分的浓度 定量地。 了解所研究药物的作用机制 了解金属的相互作用很重要 与蛋白质和其他成分形成复合物。 不幸的是 文献中报道的许多研究是在 由于缺乏分析方法而浓度更高 所需的灵敏度。 就铂类药物而言 是相对缓慢的动力学的一个进一步复杂化的因素 一些反应；许多已发表的研究假设 金属配合物种类及其相互转化。

Analytical methods have been developed for separation, quantitation and characterization of elements (principally metals) and their complex species (coordination compounds) present in biological samples at trace levels. In vivo and in vitro studies designed to produce biologically relevant results require the ability to separate and quantitate the metals in the low concentrations obtaining as well as the ability to relate their concentrations to the biological milieux and their components quantitatively. To understand the mechanisms of action of the drugs under study it is important to understand the interactions of the metal complexes with proteins and other constituents. Unfortunately many studies reported in the literature have been conducted at much higher concentrations for want of analytical methods with the requisite sensitivity. In the case of the platinum drugs there is a further complicating factor in the relatively slow kinetics of some of the reactions; many published studies assume that the metal complex species and their interconversions are.