MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

分析方法已经被开发出来用于定量和 金属元素及其络合形态的表征 痕量生物样品。 对正在研究的人体样本中的钒进行了分析 指挥。所获得的结果显示，在 每克样品含钒纳克的范围。非常少量的 的脑脊液样本使得可靠的、可重复性的结果很难 保证。 铂的络合物行为正在研究几种不同的 临床上使用的含铂的复杂化合物。色谱学 使用技术来分离铂络合物物种(结合 且未与蛋白质结合)在临床使用浓度下的人体血浆中 级别。物质平衡表明，所有铂金中的几个 分数可以用数量来说明。分析方法 已被用来对铂的行为进行动力学研究 来自顺铂的物种(包括母体复合体， 顺式二氨基二氯铂(CDDP)及其转化产物) 并将它们与每一组分中蛋白质的定量联系起来 收好了。电热雾化原子吸收光谱 用于对总铂或单个分离物种进行定量。

Analytical methods have been developed for quantitation and characterization of metallic elements and their complex species present in biological samples of trace levels. Analysis of vanadium in samples from human subjects under study has been conducted. Results obtained have shown very low concentrations, in the range of nanograms-of-vanadium-per-gram of sample. The very small amounts of CSF samples available make reliable, reproducible results difficult to assure. Platinum complex behavior is under study for several different platinum-containing complex compounds used clinically. Chromatographic techniques are used to make separations of platinum complex species (bound and unbound to protein) in human plasma at clinically used concentration levels. Material balances suggest that all the platinum in the several fractions can be accounted for quantitatively. Analytical methods developed have been used to make kinetic studies of behavior of platinum species from cisplatin (including the parent complex, cis-diamminedichloroplatinum II (CDDP), and its transformation products) in plasma and to relate them to quantitation of protein in each fraction collected. Atomic Absorption Spectroscopy with electrothermal atomization is used to quantitate total platinum or single isolated species.