MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

生物环境中痕量金属和复合物的测量

• 批准号: 3978325
• 负责人: A F LEROY
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3978325
• 关键词: analytical method; atomic absorption spectrometry; biotransformation; chromatography; cis platinum compound; clinical chemistry; high performance liquid chromatography; human tissue; metal complex; plasma; platinum; trace elements; vanadium

Analytical methods have been developed for quantitation and characterization of metallic elements and their complex species present in biological samples of trace levels. Analysis of vanadium in samples from human subjects under study has been conducted. Results obtained have shown very low concentrations, in the range of nanograms-of-vanadium-per-gram of sample. The very small amounts of CSF samples available make reliable, reproducible results difficult to assure. Platinum complex behavior is under study for several different platinum-containing complex compounds used clinically. Chromatographic techniques are used to make separations of platinum complex species (bound and unbound to protein) in human plasma at clinically used concentration levels. Material balances suggest that all the platinum in the several fractions can be accounted for quantitatively. Analytical methods developed have been used to make kinetic studies of behavior of platinum species from cisplatin (including the parent complex, cis-diamminedichloroplatinum II (CDDP), and its transformation products) in plasma and to relate them to quantitation of protein in each fraction collected. Atomic Absorption Spectroscopy with electrothermal atomization is used to quantitate total platinum or single isolated species.

已经开发了定量分析方法， 金属元素及其络合物的表征 微量生物样本 研究中的人类受试者样本中的钒分析已经 进行。 所获得的结果表明， 每克样品的钒含量范围为纳克数。 非常小的量 CSF样本的可用性使得难以获得可靠的、可重复的结果， 放心吧。 铂络合物的行为正在研究几种不同的 临床上使用的含铂络合物。 色谱 技术用于分离铂络合物物质（结合的 且未与蛋白质结合）在人血浆中的浓度 程度. 物质平衡表明，所有的铂在几个 分数可以定量计算。 分析方法 用所开发的方法对铂的行为进行了动力学研究 来自顺铂的种类（包括母体复合物， 顺二氨二氯铂II（CDDP）及其转化产物） 血浆，并将其与各组分中蛋白质的定量相关联 收集。 原子吸收分光光度法 用于定量总铂或单个分离物质。