MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

生物环境中痕量金属和复合物的测量

• 批准号: 4705632
• 负责人: A F LEROY
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4705632
• 关键词: analytical method; atomic absorption spectrometry; chromatography; cis platinum compound; high performance liquid chromatography; human tissue; metal complex; platinum; vanadium

Analytical methods have been developed for quantitation and characterization of metallic elements in biological samples. Analysis of vanadium in samples from human subjects under study has been conducted. Results obtained have shown very low values, in the range of nanograms-of-vanadium-per-gram of sample. Such results are obtained only with great difficulty because of the very samll amount of sample available. Specific interest in platinum complex behavior of several different compounds used clinically has been studied. Chromatographic techniques are used to make separations of species in solutions. Atomic Absorption spectroscopy with electrothermal atomization (ETA-AAS) is used to quantitate total platinum or single isolated species. HPLC separation with electrochemical detection using a modified polarographic analyzer permits quantitation of the parent CDDP complex in aqueous solutions with a detection limit of about 50 ppb. Analytical methods developed have been used to make kinetic studies of behavior of platinum species from cisplatin in solution.

已经开发了定量分析方法， 生物样品中金属元素的表征。 分析 钒在研究中的人类受试者的样品进行了。 所获得的结果显示出非常低的值，在 每克样品中含有纳克钒。 这样的结果只有在 由于样本量太少， available. 特别感兴趣的铂络合物行为的几个 已经研究了临床上使用的不同化合物。 色谱 技术用于分离溶液中的物质。 原子 本文采用原子吸收光谱法（ETA-AAS）， 以定量总铂或单个分离的物质。 HPLC分离 用改进的极谱分析仪进行电化学检测 允许在水溶液中定量母体CDDP复合物， 检测限约为50 ppb。 开发的分析方法已 用于对顺铂中铂类的行为进行动力学研究 在溶液中。