ENZYME PURIFICATION--CALMODULIN

酶纯化--钙调蛋白

• 批准号: 3978326
• 负责人: A F LEROY
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3978326
• 关键词: analytical chemistry; calcium; reagent /indicator; solvents

Validity of studies characterizing and measuring properties of calmodulin depend on having quantifiable measures of purity of the enzyme preparation and the proportion of calcium it contains and requires the availability of extremely pure reagents, buffer, and solvent and contamination free labware. Analytical methods have been developed for quantitation of picogram quantities of calcium in highly purified preparations of calmodulin and in the reagents and solvents needed to make the preparations. Preliminary analyses show that the needed reagents and solvents for making ultra-pure enzyme preparations can be freed to satisfactory levels from such ubiquitous contaminants as calcium using techniques developed in our laboratory.

钙调蛋白特性表征和测量研究的有效性 取决于酶制剂纯度的可定量测量 以及它所含的钙的比例， 极纯的试剂、缓冲液和溶剂以及无污染的实验室器皿。 已经开发了皮克定量的分析方法 在高度纯化的钙调蛋白制剂和 制备所需的试剂和溶剂。 初步 分析表明，所需的试剂和溶剂，使超纯 酶制剂可以从这些物质中释放到令人满意的水平， 普遍存在的污染物，如钙，使用我们开发的技术 实验室