NEUROREGULATORS AND ALCOHOL ABUSE

衍生自生物胺与 醛在酒精使用方面引起了相当大的兴趣， 滥用机制。 两种色酚类化合物， 从儿茶酚胺和四氢异喹啉， 衍生自吲哚胺，已经被各种研究者声称， 改变酒精偏好 此外，这些化合物中的一些具有 药理作用，包括苯二氮卓类药物的竞争 受体和对神经递质再摄取机制的影响。 我们的目标是 以确定这些化合物中的任何一种是否通常存在于 哺乳动物组织以及它们的存在是否被酒精改变 局 为此，我们一直在制定程序， 使用快速样品净化分析胺-醛缩合产物 随后用熔融离子化负离子质谱法进行化学电离， 硅胶毛细管柱。 我们制定的程序是 高度特异性，能够检测低浓度的 物质. 此外，这些程序消除了许多来源， 困扰其他调查人员的工作，他们声称， 色托啉和四氢异喹啉通常存在， 是在服用酒精后形成的。 这项工作的目标是 与酒精相关的问题，因为它试图提供 一个可靠的答案，关于这些化合物是否 通常存在于组织中，或者它们是否在酒精后形成 局

Condensation products derived from reactions between biogenic amines and aldehydes have elicited considerable interest in terms of alcohol use and abuse mechanisms. Both the tryptolines, the condensation products derived from catecholamines, and tetrahydroisoquinolines, the condensation products derived from indoleamines, have been claimed by various investigators to alter alcohol preferences. In addition, some of these compounds have pharmacological effects which include competition for the benzodiazepine receptor and effects on neurotransmitter reuptake mechanisms. Our goal is to determine whether any of these compounds are normally present in mammalian tissues and whether their presence is altered by alcohol administration. Toward this end we have been developing procedures for analyzing amine-aldehyde condensation products using rapid sample clean-up followed by chemical ionization negative ion mass spectrometry with fused silica capillary columns. The procedures which we have developed are highly specific and are able to detect low concentrations of the substances. Furthermore, these procedures eliminate many sources of artifact which plague the work of other investigators who have claimed that the tryptolines and tetrahydroisoquinolines are either normally present or are formed after alcohol administration. The goal of this work is of direct relevance to alcohol related problems because it attempts to provide a reliable answer to the question as to whether these compounds are normally present in tissues, or whether they are formed after alcohol administration.