TRANSFUSION-RELATED VIRAL INFECTIONS AND IMMUNE RESONSE

主题集中在与病毒感染和免疫反应相关的 输血。7个基础和临床的综合方案 在两个专门核心单位的协助下，研究项目旨在 调查当代输血传播肝炎的问题， 巨细胞病毒和艾滋病，以及由此对免疫系统的影响。分子 鸡传染性支气管炎输血后肝炎发病机制的研究 肝炎病毒以及非甲非乙型病毒将是 通过对输血患者的前瞻性研究和 动物模型的实验研究，也将用于研究 抗嗜肝病毒药物(项目1和2)。 献血者特异性输血后巨细胞病毒感染的免疫学机制 肾移植前输血与免疫抑制作用 将对同种异体输血和自体输血进行研究 在项目3、4和5中。与输血有关的艾滋病问题是 在病毒培养中阐述了感染的定义与 抗体阳性献血者对病毒的免疫力(项目6)和 建立治疗和预防措施的动物模型 艾滋病逆转录病毒(ARV/LAV/HTLV III)感染(项目7)。具体的 擅长病毒免疫学，克隆的分子杂交分析 DNA探针，用于单克隆性细胞表面标记分析的流式细胞术 在实验细胞生物学研究中进行抗体和细胞分选。 由核心单位A和B提供给所有调查人员。 目标是防止血液传播病毒感染和 用抗病毒治疗控制未解决的感染。这一目标将 通过促进调查人员之间的互动和通过使用 美国加州大学医院的临床资源 位于旧金山的锡安医疗中心和欧文纪念血库。 该项目是对加州大学旧金山分校学术承诺的补充和加强 教师在输液教学、研究和服务方面的卓越表现 医药。

The theme focuses on viral infections and immune responses associated with transfusion of blood. An integrated program of 7 basic and clinical research projects, aided by two specialized core units, is designed to investigate the contemporary problems of transfusion-transmitted hepatitis, CMV, and AIDS, and resulting effects on the immune system. The molecular mechanisms of pathogenesis of post-transfusion hepatitis caused by hepatitis viruses as well as the non-A, non-B viruses are to be investigated through prospective studies in transfused patients and experimental studies in animal models which will also be used in study of antiviral agents against hepatotropic viruses (Projects 1 and 2). Immunologic mechanisms in post-transfusion CMV infection, in donor-specific transfusions prior to renal transplantation and immunosuppressive effects per se of homologous versus autologous blood transfusions will be studied in Projects 3, 4 and 5. The problems of transfusion-associated AIDS are addressed in culturing the virus for definition of infection versus immunity to the virus in antibody positive blood donors (project 6) and in deriving animal models for therapeutic and preventive measures against the AIDS retrovirus (ARV/LAV/HTLV III) infection (project 7). The specific expertise in viral immunology, molecular hybridization analyses with cloned DNA probes, flow-cytometry for cell-surface marker analyses with monoclonal antibodies and cell sorting in experimental cell biology studies are made available to all investigators by the Core Units A and B. The ultimate goal is to prevent transmission of the blood borne viral infection and managing the unresolved infection with antiviral therapy. This goal will be achieved by promoting interaction between the investigators and by using the clinical resources of the University of California Hospitals, the Mount Zion Medical Center and the Irwin Memorial Blood Bank in San Francisco. The program complements and strengthens the academic commitment of UCSF faculty to excellence in teaching, research and service in transfusion medicine.