TRANSFUSION-RELATED VIRAL INFECTIONS AND IMMUNE RESONSE

输血相关的病毒感染和免疫反应

• 批准号: 3098542
• 负责人: GIRISH N VYAS
• 金额: $ 84.6万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3098542
• 关键词: AIDS; Betaherpesvirinae; duck hepatitis B virus; molecular biology; virus infection mechanism

The theme focuses on viral infections and immune responses associated with transfusion of blood. An integrated program of 7 basic and clinical research projects, aided by two specialized core units, is designed to investigate the contemporary problems of transfusion-transmitted hepatitis, CMV, and AIDS, and resulting effects on the immune system. The molecular mechanisms of pathogenesis of post-transfusion hepatitis caused by hepatitis viruses as well as the non-A, non-B viruses are to be investigated through prospective studies in transfused patients and experimental studies in animal models which will also be used in study of antiviral agents against hepatotropic viruses (Projects 1 and 2). Immunologic mechanisms in post-transfusion CMV infection, in donor-specific transfusions prior to renal transplantation and immunosuppressive effects per se of homologous versus autologous blood transfusions will be studied in Projects 3, 4 and 5. The problems of transfusion-associated AIDS are addressed in culturing the virus for definition of infection versus immunity to the virus in antibody positive blood donors (project 6) and in deriving animal models for therapeutic and preventive measures against the AIDS retrovirus (ARV/LAV/HTLV III) infection (project 7). The specific expertise in viral immunology, molecular hybridization analyses with cloned DNA probes, flow-cytometry for cell-surface marker analyses with monoclonal antibodies and cell sorting in experimental cell biology studies are made available to all investigators by the Core Units A and B. The ultimate goal is to prevent transmission of the blood borne viral infection and managing the unresolved infection with antiviral therapy. This goal will be achieved by promoting interaction between the investigators and by using the clinical resources of the University of California Hospitals, the Mount Zion Medical Center and the Irwin Memorial Blood Bank in San Francisco. The program complements and strengthens the academic commitment of UCSF faculty to excellence in teaching, research and service in transfusion medicine.

该主题的重点是病毒感染和免疫反应相关的 输血。 7个基础和临床的综合方案 研究项目，由两个专门的核心单位的援助，旨在 调查输血传播肝炎的当代问题， CMV和艾滋病，以及对免疫系统的影响。 分子 输血后肝炎的发病机制 肝炎病毒以及非甲、非乙型病毒将被 通过对输血患者的前瞻性研究进行了调查， 动物模型的实验研究，也将用于研究 抗嗜肝病毒的抗病毒剂（项目1和2）。 输血后巨细胞病毒感染的免疫机制，在供体特异性 肾移植前输血和免疫抑制作用 将研究同种异体输血与自体输血的比较 项目3、4、5。 输血相关艾滋病的问题是 在培养病毒以定义感染与 抗体阳性献血者对病毒的免疫力（项目6）和 获得动物模型用于治疗和预防 艾滋病逆转录病毒（ARV/LAV/HTLV III）感染（项目7） 具体 在病毒免疫学、分子杂交分析和克隆 DNA探针，流式细胞术用于单克隆抗体的细胞表面标记分析 在实验细胞生物学研究中进行抗体和细胞分选 由核心单元A和B提供给所有研究者。 最终 目的是防止血液传播病毒感染的传播， 通过抗病毒治疗来管理未解决的感染。 这一目标将 通过促进调查人员之间的互动和使用 加州大学医院的临床资源， 锡安医疗中心和弗朗西斯科的欧文纪念血库。 该计划补充和加强了UCSF的学术承诺 在输血教学、研究和服务方面取得卓越成就的教师 药