EFFECTIVENESS OF HTLV-III ANTIBODY SCREENING

本计画的目标是发展一套流式细胞仪检测系统 （FCDS）用于感染HIV-1的人淋巴细胞。 最佳条件 这些都是建立起来的，它们不仅是建立起来的， 细胞内HIV的存在，但也导致 感染艾滋病病毒。 此外，一抗和二抗反应 也被定性。 一抗对p24具有特异性， 第二抗体由A的f（ab ′）2片段组成， 荧光素缀合的山羊抗小鼠免疫球蛋白抗体。 的 该系统灵敏度足以检测出万分之一的艾滋病毒感染细胞 在实验感染的人T淋巴细胞系中未感染的细胞。 FCDS目前被用来检查已知的人的样本， 艾滋病毒感染的高风险（血友病患者及其性行为） 从随机选择的献血者。 该系统还 已被修改，包括使用leu M3藻蓝蛋白标记的 单克隆抗体（CD 14）用于鉴定感染的单核细胞。 低 在Leu M3中检测到明显阳性细胞水平 阴性细胞。 目前正在进行实验，以确定是否 这些细胞是否感染了艾滋病病毒

The goal of this project is to develop a flow cytometry detection system (FCDS) for human lymphocytes infected with HIV-1. Optimal conditions for fixation have been established; these not only delineate the presence of intracellular HIV, but also result in inactivation of infectious HIV. Furthermore, primary and secondary antibody reactions have also been characterized. The primary antibody is specific for p24, and the secondary antibody consists of the f(ab')2 fragment of a fluorescein-conjugated, goat anti-mouse immunoglobulin antibody. The system is sensitive enough to detect one HIV-infected cell in 10,000 uninfected cells in experimentally infected human T-lymphoid cell lines. The FCDS is currently being used to examine samples from people known to be at high risk of HIV infection (hemophiliacs and their sexual partners) and from randomly selected blood donors. The system has also been modified to include the use of leu M3 phycoerythrin-labelled monoclonal antibody (CD14) for identifying infected monocytes. Low levels of apparently positive cells have been detected in the leu M3 negative cells. Experiments are currently under way to identify whether or not these cells are infected with HIV.