MECHANICAL, THERMAL AND OPTICAL SIGNS OF EXCITATION IN THE NERVOUS SYSTEM

神经系统兴奋的机械、热和光学迹象

• 批准号: 3968475
• 负责人: I TASAKI
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3968475
• 关键词: action potentials; afferent nerve; axon; clinical biomedical equipment; drug metabolism; electrophysiology; fresh water environment; membrane activity; membrane permeability; membrane structure; nerve; neural conduction; neural transmission; neurochemistry; neuropharmacology; neurophysiology; nuclear magnetic resonance spectroscopy; polymerization; radiotracer; retina; spinal cord; tetraethylammonium compound; thermometry; thermoreceptors; veratrum alkaloid

(1) Improvement of Thermal Detector. Several years ago, we devised a sensitive detector for measuring small temperature changes in nervous tissues associated with their enhanced physiological activities. During the last fiscal year, we have greatly improved the signal-to-noise ratio of our thermal detector. It is now possible to apply our detector to various nervous tissues in which heat production during activity has nerver been detected. (2) Analysis of Sensory Processes in Frog Retina by Taking Heat Production as an Index. Our analysis of physiological events in the dark-adapted frog retina by using our thermal detector was initiated in Martch of 1985. Taking advantage of the improvement of our thermal detector, heat production in the retina induced by brief light stimuli was analyzed in detail. The major portion of our findings is expected to be published in the August issue of Biophysical Journal. (3) New Findings Obtained by Application of Heat Measurement Technique to Frog Spinal Cord. Using isolated bullfrog spinal cord, we succeeded in detecting heat production by the nerve cells in the cord following stimulation of the sensory nerve fibers. The time-resolution and the sensitivity of our thermal detector was high enough to permit a detailed analysis of the source of this heat. Our study of the effects of various pharmacological agents on the process of heat production by the spinal cord is now in progress. (4) Measurement of Mechanical and Optical Changes. By using various techniques developed in this laboratory, small movements and changes in turbidity of various nervous tissues were examined in conjunction with thermal measurements.

(1)热敏探测器的改进。几年前，我们设计了一种 用于测量神经性疾病微小体温变化的灵敏探测器 与增强的生理活动相关的组织。在.期间 在上一财年，我们大大提高了信号的信噪比 我们的热探测器。现在可以将我们的探测器应用于各种 在活动过程中产生热量的神经组织 检测到。(2)青蛙视网膜受热后感觉过程的分析 将生产作为一种指数。我们对生理事件的分析 使用我们的温度探测器的暗适应青蛙视网膜是在 1985年的马奇。利用我们的热性能的提高 探测器，短暂的光刺激在视网膜中诱导的热产生 对此进行了详细的分析。我们发现的主要部分预计将是 发表在8月号的《生物物理杂志》上。(3)新发现 应用热量测量技术对青蛙脊髓进行了测量。 利用分离的牛蛙脊髓，我们成功地探测到了热量 刺激脊髓中的神经细胞产生 感觉神经纤维。我们的时间分辨率和灵敏度 热探测器的高度足以详细分析 这种热量的来源。我们对不同药理作用的研究 脊髓产生热量的过程中的试剂现在正在 进步。(4)机械和光学变化的测量。通过使用 在这个实验室里开发的各种技术，小动作和 检查了不同神经组织的浊度变化。 与热测量相结合。