MOLECULAR BIOLOGY OF VARICELLA-ZOSTER VIRUS INFECTIONS

我们的主要实验室专注于分子生物学的研究， 水痘-带状疱疹病毒（VZV）的潜伏期仍在继续。 月的更新将升级服务器 水痘带状疱疹病毒DNA株的更精细的核酸内切酶图谱以及 完成了58个病毒编码转录本的初步图谱。 我们正在使用 我们广泛的VZV DNA重组体库，以绘制各种VZV编码的 基因产物 利用标记救援技术，我们试图绘制 与VZV抗病毒药物抗性相关的遗传位点。 由混合 病毒RNA的选择和体外翻译以及免疫沉淀 通过多克隆和单克隆抗体， 主要的病毒蛋白。 通过转化胸苷激酶缺陷型 小鼠L细胞，我们已经确定并绘制了负责 VZV嘧啶激酶 在未来的一年里，我们计划启动 人组织中潜伏性水痘带状疱疹病毒DNA和RNA的原位杂交研究 序列的

Our major laboratory focus on the study of the molecular biology and latency of varicella-zoster virus (VZV) has continued. We are performing finer endonuclease mapping of varicella zoster virus DNA strains as well as completing a preliminary map of 58 viral encoded transcripts. We are using our extensive library of VZV DNA recombinants to map various VZV encoded gene products. Using marker rescue techniques we are attempting to map the genetic loci associated with VZV resistance to antiviral drugs. By hybrid selection and in vitro translation of viral RNAs and immunoprecipitation with polyclonal and monoclonal antibodies we are identifying and mapping major viral proteins. By transformation of thymidine kinase deficient mouse L cells we have identified and mapped the gene responsible for the VZV pyrimidine kinase enzyme. During the coming year we plan to initiate in situ hybridization studies of human tissues for latent VZV DNA and RNA sequences.