MOLECULAR BIOLOGY OF VARICELLA-ZOSTER VIRUS INFECTIONS

水痘带状疱疹病毒感染的分子生物学

• 批准号: 4688541
• 负责人: S E STRAUS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688541
• 关键词: L cell; cell transformation; chickenpox; genetic library; genetic manipulation; genetic mapping; latent virus infection; molecular biology; shingles; varicella zoster virus; virus DNA; virus RNA; virus classification; virus genetics

Our major laboratory focus on the study of the molecular biology and latency of varicella-zoster virus (VZV) has continued. We are performing finer endonuclease mapping of varicella zoster virus DNA strains as well as completing a preliminary map of 58 viral encoded transcripts. We are using our extensive library of VZV DNA recombinants to map various VZV encoded gene products. Using marker rescue techniques we are attempting to map the genetic loci associated with VZV resistance to antiviral drugs. By hybrid selection and in vitro translation of viral RNAs and immunoprecipitation with polyclonal and monoclonal antibodies we are identifying and mapping major viral proteins. By transformation of thymidine kinase deficient mouse L cells we have identified and mapped the gene responsible for the VZV pyrimidine kinase enzyme. During the coming year we plan to initiate in situ hybridization studies of human tissues for latent VZV DNA and RNA sequences.

我们的主要实验室专注于分子生物学和 水痘-带状疱疹病毒(VZV)的潜伏期仍在继续。我们正在表演 水痘带状疱疹病毒DNA株的更精细的内切酶图谱 完成了58个病毒编码转录本的初步地图。我们正在使用 我们丰富的VZV DNA重组子文库用于定位不同编码的VZV 基因产品。使用标记营救技术，我们正试图绘制出 与VZV抗病毒药物耐药相关的基因座。通过混血 病毒RNA的筛选、体外翻译和免疫沉淀 利用多克隆和单抗，我们正在识别和绘制 主要的病毒蛋白。胸苷激酶缺陷症的转化 我们已经鉴定并定位了小鼠L细胞中与此相关的基因。 VZV嘧啶激活酶。在来年，我们计划启动 人体组织潜伏VZV DNA和RNA的原位杂交研究 序列。