MOLECULAR BIOLOGY OF VARICELLA-ZOSTER VIRUS INFECTIONS

水痘带状疱疹病毒感染的分子生物学

• 批准号: 3809650
• 负责人: S E STRAUS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809650
• 关键词: RNA directed DNA polymerase; gene expression; genetic manipulation; genetic mapping; genetic transcription; human tissue; latent virus infection; molecular biology; molecular cloning; molecular pathology; nucleic acid hybridization; open reading frames; polymerase chain reaction; shingles; synthetic nucleic acid; varicella zoster virus; virus DNA; virus genetics; virus infection mechanism; virus protein

This project constitutes one of the two major laboratory research focuses of this section. The goals are to identify, map and characterize VZV genes and proteins expressed during active and latent infections, and to define the temporal sequence and control of gene expression. To date, we mapped the viral genome, its transcripts, and a number of important genes. We proved that VZV remains latent in satellite cells of human nerve ganglia and that 4 to 11 of 72 VZV genes are expressed during latency. To better detect and characterize latency transcripts, we are currently extracting RNA from human ganglia, processing it with reverse transcriptase, and performing polymerase chain reactions (PCR) using synthetic olio nucleotides representing targeted VZV open reading frames. In other studies, we are defining the roles and components of open reading frames responsible for VZV gene regulation. Specifically, by creating mutations within genes 4 and 61, we identified protein domains responsible for gene transactivation and suppression.

这个项目构成了两个主要的实验室研究重点之一