INHALATION CARCINOGENESIS OF ENVIRONMENTAL AGENTS

本项目由五个实验项目组成：1)开发 将尝试建立吸入性苯致癌的动物模型 通过使大鼠和小鼠接触不同的苯蒸气和通过 苯与已知致白血病药物的联合暴露；2)发展 关于吸入砷化合物致癌的动物模型的研究将是 通过将大鼠暴露于三氧化二砷气雾剂和 二氧化硫和通过气管内注入波尔多的成分 3)纤维长度对石棉致癌性的影响 将通过让老鼠接触两种不同长度的石棉纤维来进行研究 它们具有相同的空气动力学质量中值直径。肺的发育 在暴露过程中将在体内监测损害：4) 研究HCl+HCHO气氛与DNA的相互作用 S-1核酸酶测定法测定皮肤和鼻腔组织中的DNA损伤 表皮细胞以及细菌；以及，5)BCME和其他 已知的致癌环境中的烷基化试剂将是 通过气相色谱和质谱学技术测定。

This program project consists of five experimental projects: 1) the development of an animal model for the carcinogenesis of inhaled benzene will be attempted by treating rats and mice to various exposure regimes of benzene vapor and by combining exposure of benzene with known leukemogenic agents; 2) the development of an animal model for the carcinogenesis of inhaled arsenic compounds will be attempted by exposing rats to a combination of arsenic trioxide aerosol and sulfur dioxide and by intratracheally instilling the components of Bordeaux mixture into rats; 3) the effect of fiber length on asbestos carcinogenicity will be studied by exposing rats to two different lengths of asbestos fibers which have the same aerodynamic mass median diameters. The development of lung damage will be monitored in vivo during the course of the exposures: 4) the interaction of atmospheres of HCl plus HCHO with DNA will be studied by measuring DNA damage by means of the S-1 nuclease assay in skin and nasal epidermal cells, as well as bacteria; and, 5) the amount of BCME and other alkylating agents in known, tumorigenic atmospheres of HCl plus HCHO will be determined by gas chromatographic and mass spectrometric techniques.