OPIOIDS AND ORGAN PRESERVATION

这个项目研究了人体的生理效应和潜在的 Delta阿片肽和a的作用生化机制 器官相关蛋白因子HIT(冬眠诱导触发) 保存和保护。HIT(休眠感应触发)，a 从冬眠土拨鼠中提取部分纯化的蛋白质因子 的成分和浓度差异进行比较 来自夏季活动动物的等量蛋白质制剂。A 88 KDa 因此，FPLC鉴定和纯化了HIT的特异性蛋白质，并 十二烷基硫酸钠/页。经Western blotting后，将纯化的蛋白进行 蛋白水解性切割。胰酶片段的序列表明 该蛋白质不属于任何已知的蛋白质，但代表一种 一种新的冬眠专用蛋白质。

This project examines the physiological effects and the underlying biochemical mechanisms of the action of the delta opioid peptides and a related protein factor HIT (Hibernation Induction Trigger) in organ preservation and protection. HIT (Hibernation Induction Trigger), a partially purified protein factor from winter hibernating woodchucks were compared for compositional and concentration differences with a equivalent protein preparation from summer-active animals. A 88 KDa protein specific to the HIT was thus identified and purified by FPLC and SDS/PAGE. After Western blotting, the purified protein was subjected to proteolytic cleavage. The sequences of the tryptic fragments indicated that the protein does not belong to any known proteins but represents a new hibernation-specific protein.