OPIOIDS AND ORGAN PRESERVATION

阿片类药物和器官保存

• 批准号: 5201692
• 负责人: T P SU
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201692
• 关键词: SDS polyacrylamide gel electrophoresis; biochemistry; cryopreservation; endogenous opioid; evaluation /testing; hibernation; high performance liquid chromatography; marmots; physiology; protein purification; protein sequence; tissue /organ preservation; western blottings

This project examines the physiological effects and the underlying biochemical mechanisms of the action of the delta opioid peptides and a related protein factor HIT (Hibernation Induction Trigger) in organ preservation and protection. HIT (Hibernation Induction Trigger), a partially purified protein factor from winter hibernating woodchucks were compared for compositional and concentration differences with a equivalent protein preparation from summer-active animals. A 88 KDa protein specific to the HIT was thus identified and purified by FPLC and SDS/PAGE. After Western blotting, the purified protein was subjected to proteolytic cleavage. The sequences of the tryptic fragments indicated that the protein does not belong to any known proteins but represents a new hibernation-specific protein.

这个项目研究了生理效应和潜在的 δ阿片肽作用的生化机制和 器官中相关蛋白因子HIT（冬眠诱导触发因子） 保存和保护。 HIT（休眠感应触发器），a 从冬眠土拨鼠中提取部分纯化的蛋白因子， 比较成分和浓度差异， 从夏季活跃的动物中提取的等量蛋白质。 A 88 KDa 因此，通过FPLC鉴定并纯化HIT特异性蛋白， SDS/PAGE。 在Western印迹之后，对纯化的蛋白进行免疫印迹。 蛋白水解裂解。 胰蛋白酶片段的序列表明， 该蛋白质不属于任何已知的蛋白质，而是代表一种 一种新的冬眠特异性蛋白