OPIOIDS AND ORGAN PRESERVATION

阿片类药物和器官保存

• 批准号: 6161719
• 负责人: T P SU
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161719
• 关键词: endogenous opioid; heart function; heart preservation; heart transplantation; laboratory rabbit; lung; lung transplantation; myocardial ischemia /hypoxia; nonhuman therapy evaluation; perfusion; respiratory function; tissue /organ preservation

This project examines the physiological effects and the underlying biochemical mechanisms of action of delta opioid peptides and a related protein HIT (Hibernation Induction Trigger) in organ preservation and protection. Delta opioid peptide DADLE dramatically enhanced the hypothermic preservation of isolated rat lungs. Compared to controls using Euro-Collins solution which developed pulmonary edema and hemorrhage, the DADLE-preserved lungs showed good flow, almost normal vascular resistance and good oxygenation. In isolated rabbit heart preparation in which hearts were subjected to 18 hrs of global ischemia, a 15 min preconditioning of the heart with DADLE was found to greatly enhance the functional recovery of myocardium. We are currently investigating if this action of DADLE is mediated via any specific subtype of delta opioid receptors and examining if this tissue protective property of DADLE can be seen in the central nervous system.

这个项目研究了生理效应和潜在的 δ阿片肽作用的生化机制和相关的 蛋白HIT（冬眠诱导触发剂）在器官保存中的应用， 保护 δ阿片肽DADLE显著增强了 离体大鼠肺的低温保存。 与对照组相比 使用Euro-Collins溶液，出现肺水肿， 出血，DADLE保存的肺显示良好的血流，几乎正常 血管阻力和良好的氧合。 离体兔心 制备，其中心脏经受18小时的整体灌注， DADLE预处理15 min， 大大促进心肌功能的恢复。 我们 目前正在研究DADLE的这种作用是否通过任何 δ阿片受体的特定亚型，并检查该组织是否 DADLE的保护特性可以在中枢神经系统中看到。