OPIOIDS AND ORGAN PRESERVATION

这个项目研究了人体的生理效应和潜在的 Delta阿片肽的生化作用机制及相关的 蛋白质HIT(冬眠诱导触发)在器官保存和治疗中的作用 保护。Delta阿片肽DADLE显著增强 大鼠离体肺的低温保存。与控件相比 使用Euro-Collins溶液会导致肺水肿和 出血，DADLE保存的肺血流良好，基本正常 血管阻力和良好的氧合能力。在离体兔心脏中 将心脏置于18小时的全球范围内 缺血，用DADLE对心脏进行15分钟的预适应 从而大大促进心肌功能的恢复。我们是 目前正在调查DADLE的这一行为是否通过任何 三角洲阿片受体的特定亚型并检查该组织 DADLE的保护作用可以在中枢神经系统中看到。

This project examines the physiological effects and the underlying biochemical mechanisms of action of delta opioid peptides and a related protein HIT (Hibernation Induction Trigger) in organ preservation and protection. Delta opioid peptide DADLE dramatically enhanced the hypothermic preservation of isolated rat lungs. Compared to controls using Euro-Collins solution which developed pulmonary edema and hemorrhage, the DADLE-preserved lungs showed good flow, almost normal vascular resistance and good oxygenation. In isolated rabbit heart preparation in which hearts were subjected to 18 hrs of global ischemia, a 15 min preconditioning of the heart with DADLE was found to greatly enhance the functional recovery of myocardium. We are currently investigating if this action of DADLE is mediated via any specific subtype of delta opioid receptors and examining if this tissue protective property of DADLE can be seen in the central nervous system.