FIBER OPTIC SENSOR FOR ENVIRONMENTAL PSEUDOESTROGENS

描述(改编自申请人的摘要)：答复 提高对雌激素模拟物的可疑作用的认识 生殖障碍和癌症，在第一阶段工作中， 研究人员论证了开发光纤的可行性 用于筛选雌激素模拟物的传感器。仪器报告速度很快 生物大分子与其相互作用的实时数据 配基。对几种雌激素和模拟物进行传感器测量 与传统的亲和力测量有很好的相关性 化合物。通过组合从两种不同类型获得的信息 对于纤维，传感器区分强雌激素和弱雌激素 在雌激素激动剂和雌激素拮抗剂之间，反映 传感器结果与生物活性之间有很强的一致性。一项专利和 这项工作的结果是正在筹备三份出版物。 拟议的第二阶段工作将包括：1)优化化学品和 分子生物学方法和方案，2)改进 数学方法，3)原型设计和施工，4) 模拟雌激素的盲样验证。由此产生的 仪器和稳定试剂提供快速评估 样品的雌激素和内分泌干扰潜力。这种方法 可适用于药物开发和鉴定的非 具有内分泌干扰特性的雌激素化合物。 建议的商业应用： 环境雌激素模拟和筛选的监测机构 用于雌激素内分泌干扰潜能的化合物，工业 监测自己的废物流中是否有伪雌激素、药物 通过筛选化合物寻找抗乳腺癌药物的公司 与雌激素受体结合或确定实时结合 研究人员研究生物分子的物质常数 信号转导机制。

DESCRIPTION (Adapted from applicant's abstract): In response to increasing awareness of the suspected role of estrogen mimics in reproductive disorders and cancer, during Phase I work, the investigators demonstrated the feasibility of developing a fiber optic sensor for screening estrogen mimics. The instrument reports rapid real-time data on binding between biological macromolecules and their ligands. Sensor measurements performed on several estrogens and mimics were well correlated with conventional affinity measurements for those compounds. By combining information obtained from two different types of fibers, the sensors distinguished between strong and weak estrogens and between estrogenic agonists and an estrogenic antagonist, reflecting strong agreement between sensor results and bioactivity. A patent and three publications are in preparation as a result of this work. Proposed Phase II work will entail: 1) optimization of chemical and molecular biological methods and protocols, 2) refinements in mathematical methods, 3) prototype design and construction, and 4) validation with blind samples of estrogen mimics. The resulting instrument and stabilized reagents provide a rapid assessment of estrogenic and endocrine disrupting potential of samples. This approach may be adapted to pharmaceutical development and identification of non- estrogenic compounds with endocrine-disrupting properties. PROPOSED COMMERCIAL APPLICATION: Agencies monitoring environmental estrogen mimics and screening compounds for estrogenic endocrine disrupting potential, industries monitoring their own waste streams for pseudo-estrogens, pharmaceutical companies seeking anti-breast cancer agents by screening compounds for binding to the estrogen receptor or determining real time binding constants of substances for biomolecules, researchers studying mechanisms of signal transduction.