FIBER OPTIC SENSOR FOR ENVIRONMENTAL PSEUDOESTROGENS

用于环境假雌激素的光纤传感器

• 批准号: 2882837
• 负责人: Judith L. Erb
• 金额: $ 32.28万
• 依托单位: IA, INC.
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-20 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2882837
• 关键词: bioassay; biomedical equipment development; biosensor device; environmental toxicology; estrogen analog; estrogen receptors; fiber optics; receptor binding

DESCRIPTION (Adapted from applicant's abstract): In response to increasing awareness of the suspected role of estrogen mimics in reproductive disorders and cancer, during Phase I work, the investigators demonstrated the feasibility of developing a fiber optic sensor for screening estrogen mimics. The instrument reports rapid real-time data on binding between biological macromolecules and their ligands. Sensor measurements performed on several estrogens and mimics were well correlated with conventional affinity measurements for those compounds. By combining information obtained from two different types of fibers, the sensors distinguished between strong and weak estrogens and between estrogenic agonists and an estrogenic antagonist, reflecting strong agreement between sensor results and bioactivity. A patent and three publications are in preparation as a result of this work. Proposed Phase II work will entail: 1) optimization of chemical and molecular biological methods and protocols, 2) refinements in mathematical methods, 3) prototype design and construction, and 4) validation with blind samples of estrogen mimics. The resulting instrument and stabilized reagents provide a rapid assessment of estrogenic and endocrine disrupting potential of samples. This approach may be adapted to pharmaceutical development and identification of non- estrogenic compounds with endocrine-disrupting properties. PROPOSED COMMERCIAL APPLICATION: Agencies monitoring environmental estrogen mimics and screening compounds for estrogenic endocrine disrupting potential, industries monitoring their own waste streams for pseudo-estrogens, pharmaceutical companies seeking anti-breast cancer agents by screening compounds for binding to the estrogen receptor or determining real time binding constants of substances for biomolecules, researchers studying mechanisms of signal transduction.

描述（改编自申请人摘要）：响应 越来越多的人认识到雌激素模拟物在 生殖系统疾病和癌症，在第一阶段的工作， 研究人员证明了开发光纤的可行性， 用于筛选雌激素模拟物的传感器。 该仪器报告迅速 生物大分子与它们之间结合的实时数据 配体。 对几种雌激素和模拟物进行的传感器测量 与传统的亲和力测量有很好的相关性， 化合物. 通过结合从两种不同类型的 在纤维中，感应器区分强雌激素和弱雌激素 以及雌激素激动剂和雌激素拮抗剂之间的关系， 传感器结果与生物活性之间的高度一致。 专利和 由于这项工作，正在编写三份出版物。 拟议的第二阶段工作将包括：1）优化化学品和 分子生物学方法和方案，2） 数学方法，3）原型设计和建造，以及4） 雌激素模拟物的盲样验证。 所得 仪器和稳定试剂提供了快速评估 样品的雌激素和内分泌干扰潜力。 这种方法 可以适用于药物开发和非药物的鉴定。 具有内分泌干扰特性的雌激素化合物。 拟定商业应用： 监测环境雌激素模拟物和筛选的机构 具有雌激素内分泌干扰潜力的化合物，工业 监测他们自己的废物流中的假雌激素， 寻求抗乳腺癌药物的公司通过筛选化合物 结合雌激素受体或测定真实的时间结合 生物分子的物质常数，研究人员研究 信号转导机制。