NOVEL STRIP TEST FOR MEASURING OVULATION

E1-g和Pd-g、17 b-雌二醇的尿代谢产物和 孕酮，可以定义可育期，并确定发生 或排卵失败。 目前的测量需要 实验室方法，因为E1-g的含量为ng/m1， E1-g浓度的变化非常微小， 半定量 IA，Inc.取得了非常令人鼓舞的初步成果 区分排卵和非排卵的测试条的开发数据 排卵水平的E1-g使用一种创新的技术， 抗体和抗原之间的结合变成视觉上明显的颜色 变化 不像使用酶或 胶体金，这些条将不需要仪器。 我们 我建议把这项创新技术，以明确的示范， 通过开发E1-g测试条的可行性， 校正尿量的变化，以实现准确的 区分生育期和周期的其余部分。 将通过比较 在条带上的颜色变化以 温度计 该方法快速、简便、试纸条稳定 比使用酶的测试更好。 建议的商业应用 检测排卵的发生或失败，并确定 肥沃的时期将找到兽医的应用，并将提供一个OTC 本发明提供了一种用于人类计划生育和体外培养的方法， 受精 该技术可以扩展到包括广泛的 激素，药物，代谢物和毒素，提供医生办公室 筛查、家庭测试、兽医测试和现场测试到位 昂贵的实验室程序。

Measurements of E1-g and Pd-g, urinary metabolites of 17b-estradiol and progesterone, can define the fertile period and determine the occurrence or failure of ovulation. Their measurement currently requires laboratory methods because E1-g is present at ng/m1 levels and because changes in concentrations of E1-g are subtle enough to require semiquantitation. IA, Inc. has obtained very encouraging preliminary data on development of test strips which distinguish ovulatory from non- ovulatory levels of E1-g using an innovative technology which translates binding between antibody and antigen into a visually apparent color change. Unlike tests measuring ng/ml concentrations using enzymes or colloidal gold, these strips will not require instrumentation. We propose to bring this innovative technology to a clear demonstration of feasibility by developing test strips for E1-g which incorporate a means of correcting for variations in urine volume so as to achieve accurate discrimination between the fertile period and the rest of the cycle. Semiquantification will be achieved by comparing the location of the color change on the strip to a concentration scale in the manner of a thermometer. The method will be rapid, simple, and strips more stable than tests employing enzymes. PROPOSED COMMERCIAL APPLICATION Strips detecting occurrence or failure of ovulation, and defining the fertile period will find veterinary applications and will provide an OTC method which will be useful for human family planning and for in vitro fertilization. The technology can be expanded to include a wide range of hormones, drugs, metabolites and toxins, providing physician's office screening, homes testing, veterinary testing, and field testing in place of expensive laboratory procedures.