FIBER OPTIC SENSOR FOR REPRODUCTIVE HORMONES

描述（改编自申请人的摘要）：虽然它是很好的 确定了许多发生在绝经后的医学状况， 妇女与妇女地位的变化有关， 生殖激素，尤其是雌激素， 这些激素水平之间的关系，在症状， 与无症状女性相比，尚不清楚。 同样地， 人们对侧位的生理基础知之甚少， 与激素相关的影响，耐受性降低和不依从性 替代疗法 这种缺乏了解的一个因素是成本 这些激素的检测， 在女性的生殖阶段进行监控 申请人 建议通过开发成本相对较低的 光纤传感器将同时测量雌二醇， 孕酮、促卵泡激素（FSH）和促黄体激素 (LH)在血清中。 该仪器将设计用于 医生的办公室，并将提供至少与那些 在几分钟内从临床试验中获得。 在第一阶段，计划 是检验将时间敏感性 使用镧系元素螯合物的分辨荧光测定（已被 用于在阿托摩尔水平上达到灵敏度的测定， 1 x 10（-18）摩尔），具有光纤的速度和简单性 生物传感器

DESCRIPTION (Adapted from applicant's abstract): Although it is well established that many medical conditions occurring in postmenopausal women are related to changes that have occurred in levels of reproductive hormones, especially estrogen, the details of the relationships between the levels of these hormones in symptomatic as compared to non-symptomatic women are not well understood. Similarly, there is little understanding of the physiological basis for side affects, reduced tolerance and noncompliance associated with hormone replacement therapy. A factor in this lack of understanding is the cost of assaying these hormones, which has kept them from being routinely monitored as a woman passes her reproductive phases. The applicant proposes to address this situation by developing a relatively low cost fiber optic sensor that will simultaneously measure estradiol, progesterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) in serum. The instrument will be designed for use in the physician's office and will provide results at least comparable to those obtained from clinical assay within minutes. During phase I, the plan is to examine the feasibility of combining the sensitivity of time resolved fluorescence assay using lanthanide chelates (that have been used in assays that have achieved sensitivity at the level of attamoles, 1 x 10 (-18) moles) with the speed and simplicity of a fiber optic biosensor.