HORMONAL CONTROL OF LUNG DEVELOPMENT--PTHRP BIOLOGY

描述：(改编自申请人的摘要)拟进行的研究 是专门为检验以下假设而设计的：(1) 胎儿II型细胞拉伸启动转导触发PTHrP PTHrP通过以下途径促进胎肺成纤维细胞成熟 受体依赖机制；(3)PTHrP刺激 促进II型细胞的间充质细胞因子和生长因子 成熟。脂蛋白脂酶、糖皮质激素受体和 PTHrP缺陷对成纤维细胞生长因子和细胞因子的调节作用 肺组织和成纤维细胞将在mrna和蛋白质水平上进行研究。 以确定此模型中的哪些功能会受到影响。

DESCRIPTION: (Adapted from the applicant's abstract) The proposed studies are specifically designed to test the following hypotheses: (1) stretch-initiated transduction in fetal type II cells triggers PTHrP production; (2) PTHrP stimulates fetal lung fibroblast maturation by receptor-dependent mechanisms; (3) PTHrP stimulates production of mesenchymal cytokines and growth factors which promote type II cell maturation. Elaboration of lipoprotein lipase, glucocorticoid receptor, and PTHrP-regulated fibroblast growth factors and cytokines by PTHrP-deficient lung tissue and fibroblasts will be studied at the mRNA and protein levels to determine which functions are affected in this model.