MODULATION OF OLFACTORY NEURONS BY GNRH AND DOPAMINE

GNRH 和多巴胺对嗅觉神经元的调节

• 批准号: 6214771
• 负责人: RONA J DELAY
• 金额: $ 3.57万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6214771
• 关键词: Urodela; dopamine; gonadotropin releasing factor; limbic system; neural transmission; neurons; neuropharmacology; olfactions; olfactory stimulus; sodium channel; tetrodotoxin; tissue /cell culture; voltage gated channel

Hormones and neurotransmitters can modulate electrical activity of peripheral olfactory receptor neurons (Eisthen et al., 1996; Lucero et al., 1996). This suggests that the olfactory output may be subject to some form of initial processing. Analogous to the way the other sensory systems (i.e. the retina, vestibular, and gustatory systems) have been shown to modulate sensory input, olfactory information may be modified before it leaves the periphery. This project is focused on peripheral modulation of olfactory neurons by endogenous neuroactive compounds. There are many possible avenues through which internal agents could modify the activity of ORNs; the substances in the mucus bathing the dendritic ends of the cells, the neuropeptides and neurotransmitters in the olfactory epithelium, even possible feedback from the olfactory bulb. Preliminary experiments in this laboratory have shown that gonadotropin-releasing hormone (GnRH) and dopamine can modulate voltage-activated currents in Necturus ORNs. Thus both of these substances may serve as neuromodulators of olfactory neurons. The goals of this study are to identify the voltage-activated currents that are modulated by GnRH and dopamine in Necturus olfactory neurons and to characterize the second messenger systems that mediate each of these effects. In addition the effects of these two potential neuromodulators on the microvillar and ciliated olfactory neurons will be determined and compared.

激素和神经递质可以调节脑电活动， 外周嗅觉受体神经元（Amarthen等，1996; Lucero et al. 例如，1996年）的报告。 这表明嗅觉输出可能受到 某种形式的初始处理。 类似于其他感官 系统（即视网膜，前庭和味觉系统）已经被 示出为调节感觉输入，嗅觉信息可以被修改 在它离开外围之前 本项目主要研究嗅觉神经元的外周调制 由内源性神经活性化合物引起。 有很多可能的途径 通过这些内部代理可以改变ORN的活性; 粘液中的物质沐浴着细胞的树突末端， 嗅觉上皮中的神经肽和神经递质，甚至 嗅球可能的反馈 初步实验， 这个实验室已经证明，促性腺激素释放激素（GnRH） 多巴胺可以调节蜜橘属ORN中的电压激活电流。 因此，这两种物质可能作为嗅觉神经调节剂 神经元 本研究的目的是确定电压激活电流 由Necturus嗅觉神经元中的GnRH和多巴胺调节 并描述第二信使系统的特征， 这些影响。 此外，这两种潜在的影响 微绒毛和纤毛嗅觉神经元上的神经调质将 确定和比较。