CSHL GENOME CENTER--A ROUGH DRAFT OF THE MOUSE GENOME

小鼠基因组序列的确定将为了解人类基因组提供关键数据。这一过程的第一步将是确定人类基因组的草图。我们已经开发了一个小的，高度灵活的基因组中心，具有极强的信息学成分。该基础设施将使我们能够快速有效地利用现有的新型毛细管测序仪，而不会因为必须利用大型凝胶测序操作而造成重大损失。因此，我们将能够在一年内将我们的操作从目前的每周大约6000次阅读扩展到每周4.8万次阅读，然后在第三年底再次翻一番，达到每周约10万次阅读。这个斜坡率将使我们能够在3年内产生大约700兆的粗略草图序列。虽然这是正在完成，我们将开发软件，以加快完成过程。该软件将允许我们在生产中使用的一些相同的工艺流程工具应用于精加工。再加上在其他地方开发的自动编辑软件，人均完成率应该会提高5-10倍。这将使我们能够以具有成本效益和及时的方式，在项目的第二阶段完成我们现在制作的鼠标草图序列。

The determination of the mouse genome sequence will provide crucial data to understand the human genome. The first step in that process will be a determination of a rough draft of the human genome. We have developed a small, highly flexible genome center with an extremely strong informatics component. This infrastructure will allow us to rapidly and efficiently take advantage of the new capillary sequencers available, without a major loss due to a large gel-based sequencing operation that must be utilized. As a result, we will be able to scale our operation from roughly 6000 reads per week currently to 48,000 reads per week in a year and then double once again to about 100,000 reads per week by the end of the third year. This ramp rate will allow us to produce about 700 megabases of rough draft sequence over 3 years. While this is being accomplished, we will be developing software to accelerate the finishing process. This software will allow some of the same process flow tools we use in production to be applied to finishing. This, when coupled to software for automated editing developed at other sites should lead to a 5-10 fold increase in per capita finishing rates. This will allow us to finish the mouse rough draft sequence we produce now in the second phase of the project, in a cost effective, timely fashion.