SEQUENCING OF A WHEAT CHROMOSOME ARM USING ILLUMINA SEQUENCING TECHNOLOGIES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在这个项目中，我们的目标是探索从Illumina测序技术产生的数据中进行小麦和其他多倍体基因组的全基因组组装的可行性。我们已经纯化了染色体臂（3DL），并在纽约冷泉港实验室和英国基因组分析中心生成了180倍覆盖率的Illumina序列;并正在尝试使用Velvet和其他领先的NGS组装器进行组装。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In this project, we aim to explore the feasibility of performing whole genome assembly in wheat and other polyploid genomes) from data produced by Illumina sequencing technologies. We have purified a chromosome arm (3DL) and generated 180x coverage of Illumina sequence at Cold Spring Harbor Laboratory, NY, and The Genome Analysis Centre, UK; and are attempting assembly using Velvet and other leading NGS-assemblers.