MEMBRANE BASED ROLLER BOTTLE

这项拨款将启动针对膜基滚轮瓶最终商业可用性的研究，一般来说，预计膜基滚轮瓶将为生物技术行业的各个部门提供商业利益，这些部门利用细胞培养与细胞分泌产物、体外基因治疗、共培养和蛋白质纯化相关的应用。通过修改传统的滚轮瓶纳入膜为基础的属性的优势，预计是众多的和独特的每个目标应用。本提案的主要目的是准确评估膜基滚瓶原型在重组蛋白生产、逆转录病毒细胞转导和蛋白质纯化三个目标应用中的可行性。在原型的应用测试之前，必须满足几个中间目标。首先，候选膜将被表征和量化有关的具体属性。不合格的候选膜将从进一步的测试中剔除。接下来，可接受的候选膜将被构建到配置用于目标应用的原型中。将测试这些原型的各种属性，这些属性使原型能够在目标应用程序中进行测试。最后，合格的原型将在目标应用程序中进行测试。建议的商业应用：膜技术在从细胞培养到不需要物质的透析等生物技术应用中提供了好处。迄今为止，这些益处主要与悬浮细胞培养有关。然而，贴壁培养可以通过创建包含膜属性的滚轮瓶来获得这些好处。基于膜的滚轮瓶将通过提高重组蛋白的生产效率、靶细胞的转导和细胞培养应用（细胞本身是感兴趣的产物），为生物技术界带来预期的好处。

This grant will initiate research directed towards the eventual commercial availability of a membrane based roller bottle, in general, it is anticipated that a membrane based roller bottle will provide commercial benefits to various sectors within the biotechnology industry that utilize cell culture for applications related to cell secreted products, ex vivo gene therapy, co-culture, and protein purification, The advantages derived by modifying the traditional roller bottle to incorporate membrane based attributes are anticipated to be numerous and unique for each target application. The primary aim of this proposal is to accurately assess the feasibility of membrane based roller bottle prototypes in three targeted applications - recombinant protein production, retro-viral cell transduction, and protein purification. Prior to application testing of prototypes several intermediate objectives must be met. First, candidate membranes will be characterized and quantified with regard to specific attributes. Unacceptable candidate membranes will be eliminated from further testing. Next, acceptable membrane candidates will be built into prototypes configured for use in target applications. These prototypes will be tested for a variety of attributes that qualify the prototypes for testing in target applications. Lastly, the qualified prototypes will be tested in the target applications. PROPOSED COMMERCIAL APPLICATIONS: Membrane technology has provided benefits to applications in biotechnology ranging from cell culture to dialysis of unwanted material. To date, these benefits have been mainly associated with suspension cell culture. However, adherent culture could obtain these benefits by creating a roller bottle that included membrane attributes. A membrane based roller bottle would bring anticipated benefits to the biotechnology community by improving the efficiency of the production of recombinant protein, the transduction of target cell and cell culture applications where cells themselves are the product of interest.