MEMBRANE BASED ROLLER BOTTLE

描述(申请人提供)：目前进行细胞培养是为了产生少量和大量的细胞和细胞分泌的产品，如生长因子、酶、激素、单抗、干扰素、白细胞介素素、疫苗、诊断和病毒载体。各种商业上可用的基于膜的细胞培养设备可用于培养已适应悬浮培养的哺乳动物细胞。然而，越来越多的工业上重要的细胞依赖于锚定。现有的基于膜的装置缺乏合适的材料来附着细胞，并且具有次优的表面积。膜基辊瓶(MBRB)是一种实用、经济高效的培养设备，旨在满足这一需求并提高培养效率。MBRB结合了传统滚筒瓶的简单性和基于薄膜的属性。在拟议的第二阶段研究中，MBRB将针对锚定依赖细胞、细胞分泌产物和细胞转导的生产进行优化。具体目标包括彻底的机械设计调查，然后进行现场和非现场应用测试。基于膜的研究和临床规模滚筒瓶设备家族的商业化预计将导致更有效地利用生物技术资源。在第二阶段完成后，MBRB将针对中小型培养设备制造商，用于生产诊断和治疗应用的细胞或细胞产品。

DESCRIPTION (provided by applicant): The culture of cells is currently undertaken to produce small and large quantities of cells and cell-secreted products, such as growth factors, enzymes, hormones, monoclonal antibodies, interferons, interleukins, vaccines, diagnostics, and viral vectors. A variety of commercially available membrane-based cell culture devices are available for the culture of mammalian ceils that have been adapted to suspension culture. A growing number of industrially important cells, however, are anchorage-dependent. Existing membrane-based devices lack the proper materials for adherent cell attachment and have sub-optimal surface areas. The membrane based roller bottle (MBRB) is a practical, cost effective culture device designed to meet this need and improve culture productivity. The MBRB combines the simplicity of the traditional roller bottle with membrane-based attributes. In the proposed Phase II studies, the MBRB will be optimized for the production of anchorage-dependent cells, cell-secreted products, and cellular transduction. Specific aims include a thorough mechanical design investigation followed by on-site and off-site application testing. The commercialization of a membrane-based family of research and clinical scale roller bottle devices is expected to lead to a more efficient use of biotechnology resources. At the completion of Phase II, the MBRB will be targeted to manufacturers of small and mid-scale culture devices for the production of cells or cell products for diagnostic and therapeutic applications.