PRIMARY VISCERAL AFFERENTS IN PAIN

内脏引起的疼痛与各种疾病有关。 这项研究建议的主要目标是改善我们的 对内脏痛外周机制的认识 正常的生理和病理生理条件。单光纤 将进行研究以了解主要内脏的作用 胃肠道(GI)痛的传入。目标包括 大鼠肌肉和粘膜传入反应的特征 大鼠迷走神经和内脏神经对机械的、热的和 正常生理和炎症状态下的化学刺激 条件。实验将达到以下目标：1) 迷走神经和内脏神经传入的定量特征 这两条通向分级膨胀的路径将与 了解它们在调节功能和伤害性感受中的相对作用。 机械敏感感受区在胃和胃中的位置 小肠会被测定。 2)测定迷走神经和内脏神经的反应特异性 传入并确定机械不敏感的传入 支配上消化道的人真正安静，或者正在化疗和/或 温度敏感型。 3)定量表征其力学、热学和化学特性。 迷走神经和内脏神经传入神经支配的敏感性 慢性发炎的胃和小肠。 4)定量表征其力学、热学和化学特性。 迷走神经和内脏神经传入神经支配的敏感性 慢性发炎的胃和小肠。 这项建议是对 初级传入神经在上消化道疼痛中的可能作用。这项研究将 提供有关传入的适当自然刺激的信息 关于炎症过程中反应特征的变化。

Pain arising from the viscera is associated with a variety of disorders. The major goal of this research proposal is to improve our understanding of the peripheral mechanisms of visceral pain under normal physiological and pathophysiological conditions. Single fiber studies will be undertaken to understand the role of primary visceral afferents in gastrointestinal (GI) pain. The aims involve characterization of responses of muscle and mucosal afferents in the vagus and splanchnic nerves of the rat to mechanical, thermal and chemical stimuli under normal physiological and inflammatory conditions. Experiments will address the following aims: 1) Quantitative characterization of vagal and splanchnic nerve afferents in these two pathways to graded distension will be compared to understand their relative roles in regulatory functions and nociception. The locations of mechanosensitive receptive fields in the stomach and small intestine will be determined. 2) Determine response-specificity of vagal and splanchnic nerve afferents and determine whether mechanically-insensitive afferents innervating the upper GI tract are truly silent or are chemo- and/or thermo-sensitive. 3) Quantitatively characterize the mechano, thermo- and chemo- sensitive properties of vagal and splanchnic nerve afferents innervating the chronically inflamed stomach and small intestine. 4) Quantitatively characterize the mechano, thermo- and chemo- sensitive properties of vagal and splanchnic nerve afferents innervating the chronically inflamed stomach and small intestine. This proposal represents the first systematic investigation of the possible roles of primary afferents in upper GI pain. The study will provide information about the adequate natural stimuli of the afferents ad about changes in response characteristics during inflammation.