PHYSIOLOGICAL ANALYSIS OF THE OLFACTORY PROJECTIONS

描述：(改编自《调查者摘要》)：大量 编码可能的嗅觉受体蛋白的基因已经被发现，但是 如果没有合适的表达系统，激活每一个人的气味 这些受体仍然未知。作为一种将气味与特定物质联系起来的手段 受体，建议进行一系列实验来识别 最大限度地激活不同表达区域中的受体细胞 用原位杂交技术鉴定嗅觉上皮细胞 定义气味的分子特征，这些特征决定了 回应。嗅觉局部区域的EOG记录 将使用上皮细胞而不是单个受体细胞进行记录 因为找到具有相同响应的细胞的概率很低 属性。每个表达区域内的受体多样性问题 将通过使用交叉适应来选择性地抑制 由某些受体介导的反应。局部刺激 上皮细胞将被用来确定是否有上皮区 兴奋或抑制嗅球中特定类型的神经元。这个 延髓神经元的反应将通过细胞内记录和 通过染色标记对细胞进行形态鉴定。这些实验将 提供对化学结构和气味之间的关系的见解，a 感觉通道在食欲、社会行为和内分泌中的重要作用 功能。

DESCRIPTION: (Adapted From The Investigator's Abstract): A large number of genes coding putative olfactory receptor proteins have been identified, but without a suitable system for expression, the odors which activate each of these receptors remain unknown. As a means of relating odorants to specific receptors, a series of experiments are proposed to identify odors that maximally activate receptor cells in the various expression zones in the olfactory epithelium identified by in situ hybridization studies and then to define the molecular characteristics of the odors that determine the responses. EOG recordings from localized regions of the olfactory epithelium will be used instead of recording from single receptor cells because of the low probability of finding cells with identical response properties. The issue of receptor diversity within each expression zone will be approached by using cross-adaptation to selectively depress responses mediated by some receptors. Localized stimulation of the epithelium will be used to determine if there are regions of epithelium that excite or inhibit particular types of neurons in the olfactory bulb. The responses of bulbar neurons will be assessed by intracellular recording and the cells morphologically identified by dye marking. These experiments will offer insights into the relationship between chemical structure and smell, a sensory modality important in appetite, social behavior and endocrine function.