CORE--RABBIT AND HUMAN BLADDER TISSUE

核心——兔和人膀胱组织

• 批准号: 6201936
• 负责人: Stephen Anthony Zderic
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201936
• 关键词: animal genetic material tag; animal tissue; biomedical facility; human genetic material tag; human tissue; laboratory rabbit; smooth muscle; tissue resource /registry; urinary bladder; urinary bladder disorder

The Bladder Tissue Core (Core B) will be used by all three projects in the Urology Research Center. The aim of the Bladder Tissue Core is to provide the program investigators with smooth muscle tissues from normal, obstructed and reversed rabbit bladders which have been characterized physiologically. It will also provide specimens from adult patients, with well-characterized symptoms of outlet obstruction, who are treated at the University of Pennsylvania Medical Center. In addition, protein extracts and RNA will be prepared from human and rabbit samples and distributed to various investigators for analyses. The Core will perform sham operations and surgery to produce partial outlet obstruction in rabbits, provide care for the animals postoperatively, obtain biopsies from bladders to monitor the degree of bladder smooth muscle hypertrophy and functional compensation, perform reversal surgery, sacrifice animals at appropriate time points, perform basic physiological characterization of the contractility of the bladder tissue, and distribute to the bladder tissues to the participating investigators. Physiological experiments will be performed with muscle strips to determine the ability of smooth muscle samples to produce force and with whole bladders to assess bladder emptying, since this property cannot be ascertained from isometric studies alone. Tissue samples will be either used immediately or frozen in liquid nitrogen for biochemical analysis or treatment with OCT for frozen sectioning for histology and immunohistochemistry and stored at -80 degrees Celsius for future use. All available information regarding the tissues at the time of distribution, or gathered subsequently from various projects in the Urology Research Center, will be logged onto a computer and made available to the investigators in the Center. Human detrusor smooth muscle tissues will be obtained from patients whose voiding dysfunction is clearly delineated based on urodynamic work-up and will be subdivided into those patients with low versus high post-void residual urine valves. Controls will be obtained from surgical specimens from non-cancerous regions of human bladder and from bladders of patient that have prostate cancers that did not produce an obstruction. Small muscle strips from human bladder tissue samples will be used for physiological studies to assess both the isometric force generating abilities as well as velocity of shortening. Additional human tissue will be frozen to allow for isolation of protein and RNA for correlation with physiologic studies. In studying a small number of well defined human bladder smooth muscle specimens, this core will help provide a crucial link between basic research findings and the clinical condition.

膀胱组织芯（芯B）将用于所有三个项目， 泌尿外科研究中心。膀胱组织芯的目的是提供 该项目的研究人员使用了正常的平滑肌组织， 阻塞和逆转的兔膀胱， 生理上的。它还将提供成年患者的标本， 出口梗阻的特征性症状，在 宾夕法尼亚大学医学中心。此外，蛋白质提取物 和RNA将从人和兔样本中制备，并分发给 不同的研究人员进行分析。 核心将进行假手术和手术，以产生部分 出口梗阻的兔子，提供照顾的动物 术后，从膀胱获得活检以监测 膀胱平滑肌肥大和功能代偿，执行 逆转手术，在适当的时间点处死动物，进行 膀胱收缩力的基本生理学特征 组织，并将膀胱组织分配给参与者。 investigators.将用肌肉进行生理实验 测定平滑肌样品产生力的能力 并与整个膀胱评估膀胱排空，因为这个属性 不能仅从等距研究中确定。组织样本将 可以立即使用，也可以在液氮中冷冻用于生化 使用OCT进行组织学冷冻切片分析或治疗， 免疫组织化学染色，并储存在-80摄氏度以备将来使用。所有 分销时有关组织的可用信息， 或随后从泌尿学研究的各种项目中收集的 中心，将登录到一台计算机，并提供给 中心的调查员。将人逼尿肌平滑肌组织 从排尿功能障碍明确描述的患者中获得 根据尿动力学检查，并将被细分为这些患者 排尿后残余尿阀的高低。控件将被 从来自人的非癌区域的手术标本获得 膀胱和前列腺癌患者的膀胱 而不是制造障碍。人体膀胱组织的小肌肉条 样本将用于生理学研究，以评估 等长力产生能力以及缩短速度。 将冷冻额外的人体组织以分离蛋白质 和RNA与生理学研究的相关性。在研究一个小 许多明确的人膀胱平滑肌标本，这一核心 将有助于在基础研究结果与 临床状况