CORE--RABBIT AND HUMAN BLADDER TISSUE

核心——兔和人膀胱组织

• 批准号: 6346143
• 负责人: Stephen Anthony Zderic
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346143
• 关键词: animal genetic material tag; animal tissue; biomedical facility; human genetic material tag; human tissue; laboratory rabbit; smooth muscle; tissue resource /registry; urinary bladder; urinary bladder disorder

The Bladder Tissue Core (Core B) will be used by all three projects in the Urology Research Center. The aim of the Bladder Tissue Core is to provide the program investigators with smooth muscle tissues from normal, obstructed and reversed rabbit bladders which have been characterized physiologically. It will also provide specimens from adult patients, with well-characterized symptoms of outlet obstruction, who are treated at the University of Pennsylvania Medical Center. In addition, protein extracts and RNA will be prepared from human and rabbit samples and distributed to various investigators for analyses. The Core will perform sham operations and surgery to produce partial outlet obstruction in rabbits, provide care for the animals postoperatively, obtain biopsies from bladders to monitor the degree of bladder smooth muscle hypertrophy and functional compensation, perform reversal surgery, sacrifice animals at appropriate time points, perform basic physiological characterization of the contractility of the bladder tissue, and distribute to the bladder tissues to the participating investigators. Physiological experiments will be performed with muscle strips to determine the ability of smooth muscle samples to produce force and with whole bladders to assess bladder emptying, since this property cannot be ascertained from isometric studies alone. Tissue samples will be either used immediately or frozen in liquid nitrogen for biochemical analysis or treatment with OCT for frozen sectioning for histology and immunohistochemistry and stored at -80 degrees Celsius for future use. All available information regarding the tissues at the time of distribution, or gathered subsequently from various projects in the Urology Research Center, will be logged onto a computer and made available to the investigators in the Center. Human detrusor smooth muscle tissues will be obtained from patients whose voiding dysfunction is clearly delineated based on urodynamic work-up and will be subdivided into those patients with low versus high post-void residual urine valves. Controls will be obtained from surgical specimens from non-cancerous regions of human bladder and from bladders of patient that have prostate cancers that did not produce an obstruction. Small muscle strips from human bladder tissue samples will be used for physiological studies to assess both the isometric force generating abilities as well as velocity of shortening. Additional human tissue will be frozen to allow for isolation of protein and RNA for correlation with physiologic studies. In studying a small number of well defined human bladder smooth muscle specimens, this core will help provide a crucial link between basic research findings and the clinical condition.

膀胱组织核心（核心 B）将被所有三个项目使用 泌尿外科研究中心。膀胱组织核心的目的是提供 项目研究人员使用正常的平滑肌组织， 已描述的梗阻和逆转的兔膀胱 生理上。它还将提供成年患者的标本， 出口阻塞的明显症状，在医院接受治疗 宾夕法尼亚大学医学中心。此外，蛋白质提取物 RNA将从人类和兔子样本中制备并分发给 各种研究人员进行分析。 核心将进行假手术和手术以产生部分 兔子出口阻塞，照顾动物 术后，从膀胱获取活组织检查以监测膀胱的程度 膀胱平滑肌肥大和功能代偿，执行 逆转手术，在适当的时间点处死动物，进行 膀胱收缩力的基本生理特征 组织，并分布到参与的膀胱组织 调查人员。生理实验将用肌肉进行 用于确定平滑肌样本产生力的能力的条带 并用整个膀胱来评估膀胱排空，因为这种特性 不能仅通过等轴测研究来确定。组织样本将 立即使用或冷冻在液氮中用于生化 使用 OCT 进行分析或治疗，用于组织学冷冻切片和 免疫组织化学并保存在-80摄氏度以备将来使用。全部 分发时有关组织的可用信息， 或随后从泌尿学研究的各个项目中收集 中心，将登录到计算机上并可供 中心的调查员。人类逼尿肌平滑肌组织将 从排尿功能障碍明显的患者获得 基于尿动力学检查，并将被细分为这些患者 具有低与高排尿后残余尿阀。控制措施将是 从人类非癌区域的手术标本中获得 膀胱和患有前列腺癌的患者的膀胱 不产生阻碍。来自人类膀胱组织的小肌肉条 样本将用于生理研究，以评估 等长力产生能力以及缩短速度。 额外的人体组织将被冷冻以分离蛋白质 和RNA与生理学研究的相关性。在学习小 数量明确的人类膀胱平滑肌标本，这个核心 将有助于在基础研究成果和 临床状况。