CORE--RABBIT AND HUMAN BLADDER TISSUE

核心——兔和人膀胱组织

• 批准号: 6346143
• 负责人: Stephen Anthony Zderic
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346143
• 关键词: animal genetic material tag; animal tissue; biomedical facility; human genetic material tag; human tissue; laboratory rabbit; smooth muscle; tissue resource /registry; urinary bladder; urinary bladder disorder

The Bladder Tissue Core (Core B) will be used by all three projects in the Urology Research Center. The aim of the Bladder Tissue Core is to provide the program investigators with smooth muscle tissues from normal, obstructed and reversed rabbit bladders which have been characterized physiologically. It will also provide specimens from adult patients, with well-characterized symptoms of outlet obstruction, who are treated at the University of Pennsylvania Medical Center. In addition, protein extracts and RNA will be prepared from human and rabbit samples and distributed to various investigators for analyses. The Core will perform sham operations and surgery to produce partial outlet obstruction in rabbits, provide care for the animals postoperatively, obtain biopsies from bladders to monitor the degree of bladder smooth muscle hypertrophy and functional compensation, perform reversal surgery, sacrifice animals at appropriate time points, perform basic physiological characterization of the contractility of the bladder tissue, and distribute to the bladder tissues to the participating investigators. Physiological experiments will be performed with muscle strips to determine the ability of smooth muscle samples to produce force and with whole bladders to assess bladder emptying, since this property cannot be ascertained from isometric studies alone. Tissue samples will be either used immediately or frozen in liquid nitrogen for biochemical analysis or treatment with OCT for frozen sectioning for histology and immunohistochemistry and stored at -80 degrees Celsius for future use. All available information regarding the tissues at the time of distribution, or gathered subsequently from various projects in the Urology Research Center, will be logged onto a computer and made available to the investigators in the Center. Human detrusor smooth muscle tissues will be obtained from patients whose voiding dysfunction is clearly delineated based on urodynamic work-up and will be subdivided into those patients with low versus high post-void residual urine valves. Controls will be obtained from surgical specimens from non-cancerous regions of human bladder and from bladders of patient that have prostate cancers that did not produce an obstruction. Small muscle strips from human bladder tissue samples will be used for physiological studies to assess both the isometric force generating abilities as well as velocity of shortening. Additional human tissue will be frozen to allow for isolation of protein and RNA for correlation with physiologic studies. In studying a small number of well defined human bladder smooth muscle specimens, this core will help provide a crucial link between basic research findings and the clinical condition.

膀胱组织核心(核心B)将在所有三个项目中使用 泌尿外科研究中心。膀胱组织核心的目的是提供 该项目的研究人员使用的是正常、 受阻和反转的兔膀胱的特征是 生理上的。它还将提供成年患者的标本， 出口梗阻的典型症状，在 宾夕法尼亚大学医学中心。此外，蛋白质提取物 RNA将从人和兔的样本中制备并分发给 不同的调查人员进行分析。 核心将进行假手术和手术，以产生部分 兔出口梗阻，为动物提供护理 术后，从膀胱取材以监测病变程度。 膀胱平滑肌肥大与功能代偿 逆行手术，在适当的时间点处死动物，表演 膀胱收缩功能的基本生理特征 组织，并分配到参与的膀胱组织 调查人员。生理学实验将在肌肉上进行 测定平滑肌肉样本产生力量的能力的试条 并有完整的膀胱来评估膀胱的排空，因为这个属性 不能仅从等轴测研究中确定。组织样本将被 要么立即使用，要么冷冻在液氮中用于生化 组织学和组织学冷冻切片的OCT分析与治疗 免疫组织化学，并储存在零下80摄氏度以备将来使用。全 关于分发时的组织的可用信息， 或随后从泌尿学研究的各种项目中收集 中心，将登录到一台计算机并提供给 该中心的调查人员。人逼尿肌组织将被 从排尿功能障碍患者那里获得 基于尿动力学检查，将细分为那些患者 排尿后残余尿阀低位与高位。控制将是 取自人体非癌区的手术标本 从患有前列腺癌的患者的膀胱和膀胱中 不会造成障碍物。人膀胱组织的小肌条 样本将被用于生理研究，以评估 等长力的产生能力以及缩短的速度。 更多的人体组织将被冷冻，以便分离蛋白质 和与生理学研究相关的RNA。在研究一个小的 明确定义的人类膀胱平滑肌样本的数量，这个核心 将有助于在基础研究成果和 临床情况。