Laser Capture for Macromolecular Analysis of Normal Development and Pathology
用于正常发育和病理学大分子分析的激光捕获
基本信息
- 批准号:6107992
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
ort term goals include trying to infer more useful
microstructural information from our MRI signal, such as mean cell
volume, extracellular volume, intracellular volume, and membrane
permeability within a region of tissue as large as 1mm x 1mm x
1mm (using conventional MRI), and as small as 15 micron x 15
micron x 15 micron (using microscopic MRI). With Yoram Cohen,
Professor of Chemistry at Tel Aviv University, we are applying
DT-MRI to isolated optic nerve bundles, so that we can
improve our ability to view or identify structural features in these
different compartments. A recent Israel/US Bi-National
Science Foundation Grant that we applied for, and received in '98,
should help support this activity. Kimberlee Potter, who will
be working with us full-time in January 1999, will be studying
extracellular matrices, such as neocartilage tissue cultures. She
will investigate the relationship between the NMR signal and tissue
structure, with the hope that by using the NMR signal, we
will be able to follow microstructural changes in cartilage in vivo,
as we have done successfully in the brain. When Ferenc
Horkay arrives in '99, he will work on developing anisotropic
diffusion phantoms made of polymeric gels, whose properties and
structure we can control and measure using other experimental
techniques (e.g., x-ray diffraction, neutron scattering, ?). This
again will allow us to calibrate our MRI diffusion measurements,
and other performing our techniques in other Centers. More
importantly, these phantom studies will help us develop a better
understanding of the relationship between ultra and
microstructure and the NMR signal that we measure. A
collaboration with Akram Aldroubi at Vanderbilt University is
resulting in a mathematical framework for analyzing and
representing these complex diffusion tensor data sets, which are
inherently discrete, noisy, and voxel-averaged. This work is
providing continuous, smoothed tensor fields, with subvoxel
resolution. This development will allow us to apply all of the
machinery of differential geometry to study our measured tensor
fields. The main utility of this will help us follow fiber tracts as well
as introduce several new MRI "stains" that reveal architectural
features of tissues, such as its local curvature. Work with Sini
Pajevic (CIT) is resulting in a statistical framework of tensor
imaging which will permit us to answer important question using
the powerful methods of hypothesis testing, which will aid us in
quantitatively addressing issues such as whether nerve or muscle
fiber tracts are continuos, or whether different regions of the brain
are connected to each other via fiber tract pathways. Finally, with
Carlo Pierpaoli, will we continue our in vivo animal studies,
which employ DT-MRI to follow cortical development in kittens.
Clinical studies in infants may be possible under a recent
NINDS/NIMH joint RPF for pediatric neuroimaging which
identifies the intramural NIH program as a possible site for clinical
diffusion tensor MRI studies."
ort术语目标包括尝试推断出更有用的信息
来自 MRI 信号的微观结构信息,例如平均细胞
体积、细胞外体积、细胞内体积和膜
组织区域内的渗透性大至 1mm x 1mm x
1 毫米(使用传统 MRI),小至 15 微米 x 15
微米 x 15 微米(使用显微 MRI)。与约拉姆·科恩一起,
特拉维夫大学化学教授,我们正在申请
对孤立的视神经束进行 DT-MRI,这样我们就可以
提高我们查看或识别这些结构特征的能力
不同的隔间。最近以色列/美国两国
98年申请,受到的科学基金补助金,
应该有助于支持这项活动。金伯利·波特,谁会
1999 年 1 月与我们一起全职工作,将学习
细胞外基质,例如新软骨组织培养物。她
将研究 NMR 信号与组织之间的关系
结构,希望通过使用核磁共振信号,我们
将能够跟踪体内软骨的微观结构变化,
正如我们在大脑中成功所做的那样。当费伦茨
Horkay 于 99 年抵达,他将致力于开发各向异性
由聚合物凝胶制成的扩散模型,其特性和
我们可以使用其他实验来控制和测量结构
技术(例如,X 射线衍射、中子散射、?)。这
再次允许我们校准 MRI 扩散测量,
以及其他在其他中心执行我们的技术的人。更多的
重要的是,这些模型研究将帮助我们开发更好的
理解超与超之间的关系
微观结构和我们测量的核磁共振信号。一个
与范德比尔特大学 Akram Aldroubi 的合作
产生一个用于分析和分析的数学框架
表示这些复杂的扩散张量数据集,它们是
本质上是离散的、嘈杂的和体素平均的。这部作品是
提供连续、平滑的张量场,带有子体素
解决。这一发展将使我们能够应用所有
微分几何机制来研究我们测量的张量
字段。其主要用途也将帮助我们追踪纤维束
介绍几种新的 MRI“污点”,揭示建筑结构
组织的特征,例如其局部曲率。与西尼合作
Pajevic(CIT)正在产生张量的统计框架
成像,这将使我们能够使用以下方法回答重要问题
假设检验的强大方法将帮助我们
定量解决神经或肌肉等问题
纤维束是连续的,或者大脑的不同区域
通过纤维束路径相互连接。最后,与
卡罗·皮尔保利(Carlo Pierpaoli),我们会继续我们的体内动物研究吗?
采用 DT-MRI 来跟踪小猫的皮质发育。
根据最近的一项研究,婴儿的临床研究可能是可能的
NINDS/NIMH 联合 RPF 用于儿科神经影像学
将 NIH 校内项目确定为临床的可能场所
扩散张量 MRI 研究。”
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(4)
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Robert F Bonner其他文献
Cardiac pacing by shock waves during pulsed laser angioplasty
- DOI:
10.1016/0735-1097(90)91941-m - 发表时间:
1990-02-01 - 期刊:
- 影响因子:
- 作者:
Robert F Bonner;Antonio Bartorelli;Yaron Almagor;Gad Keren;Ernst Hansch;Martin B Leon - 通讯作者:
Martin B Leon
Robert F Bonner的其他文献
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{{ truncateString('Robert F Bonner', 18)}}的其他基金
LASER CAPTURE FOR MACROMOLECULAR ANALYSIS OF NORMAL DEVELOPMENT AND PATHOLOGY
用于正常发育和病理学大分子分析的激光捕获
- 批准号:
6290168 - 财政年份:
- 资助金额:
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Temporal-Spectral Control of Artificail Lighting for Improved Health
人工照明的时域光谱控制以改善健康
- 批准号:
8351258 - 财政年份:
- 资助金额:
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Laser Capture For Macromolecular Analysis Of Normal Development And Pathology
激光捕获用于正常发育和病理学的大分子分析
- 批准号:
8149233 - 财政年份:
- 资助金额:
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Temporal-Spectral Control of Artificail Lighting for Improved Health
人工照明的时域光谱控制以改善健康
- 批准号:
8553986 - 财政年份:
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Laser Capture For Macromolecular Analysis Of Normal Development And Pathology
激光捕获用于正常发育和病理学的大分子分析
- 批准号:
8351097 - 财政年份:
- 资助金额:
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Laser Capture For Macromolecular Analysis Of Development
激光捕获用于大分子分析的开发
- 批准号:
7201693 - 财政年份:
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Laser Capture For Macromolecular Analysis Of Normal Development And Pathology
激光捕获用于正常发育和病理学的大分子分析
- 批准号:
8941426 - 财政年份:
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慢性黄斑光化学损伤的光谱光保护
- 批准号:
7594233 - 财政年份:
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