Proteomics & Genomics Hands-On Workshop: From Sample Preparation to Data Analysis

描述(由申请人提供)： 近年来，基因组学和蛋白质组学已成为生物医学研究中的关键技术， 因此，热衷于在他们的研究中利用这些技术的调查人员对培训的兴趣激增。使用这些先进技术需要训练有素的人员，以产生有价值的成果、高质量的出版物和出色的赠款申请。为了发挥最大效益，理想情况下，教育应该在实验开始之前进行，以减少选择错误平台的可能性。例如，整合“组学”技术的第一步是开发一个表述良好的假设，清楚地定义正在调查的研究问题。为了检验这一假设，研究人员必须选择使用哪种平台，这需要了解可用的技术以及每种方法的优缺点。或者，研究人员可以确定基因组学和蛋白质组学方法的结合是必要的。因此，仔细的实验设计是项目成功的关键，也是解释结果的关键。鉴于这些技术产生的大量数据集，生物统计学、数据分析和生物信息学的知识至关重要，在这些领域，许多研究人员需要额外的培训，特别是在“组学”研究方面。为了解决上述问题，至少可以进行两种类型的“组学”技术培训 设想：1)以研讨会的形式立即进行短期培训，2)以研究生教育方案的形式进行长期、全面的指导。目前的建议旨在通过在一个连贯的、以实验为基础的框架内提供基因组学和蛋白质组学方面的实践培训来满足前者的需求。该计划的负责人已经开发和教授了涵盖蛋白质组学、基因组学、生物信息学和生物统计学的课程，拟议课程的课程将结合他们的专业知识，提供这些关键技术的全面培训概述。具体地说，我们建议完成以下工作：建立一个课程，使蛋白质组学和基因组技术能够以结果为结构的学习；使用实践技术和有经验的研究人员和教育工作者建立的模式提供新颖的培训经验；传播教育材料，使知识和信息能够造福于广泛的受众，并为年轻和成熟的研究人员提供和/或充当合作机会的门户。 (摘要结束)

DESCRIPTION (provided by applicant): In recent years, genomics and proteomics have emerged as key technologies in biomedical research, resulting in a surge of interest in training by investigators keen to utilize these technologies in their research. Use of these advanced technologies requires trained personnel in order to produce valuable results, quality publications, and superior grant applications. To be of maximum benefit, education should ideally occur before experiments are begun, in order to lessen the possibility that the wrong platform is chosen. For example, a first step toward integrating "omics" technologies is the development of a well-stated hypothesis which clearly defines the research question under investigation. In order to test the hypothesis, the researcher must choose which platform to utilize and this requires an understanding of the technology available and the strengths and weaknesses of each approach. Alternatively, an investigator may determine that a combination of genomics and proteomics approaches is necessary. Careful experimental design is then critical to the success of the project as well as for interpreting the results. Given the large datasets generated by these technologies, knowledge of biostatistics, data analysis, and bioinformatics are vital and these are areas in which many investigators require additional training, specifically in the context of "omics" research. To address the issues outlined above, at least two types of training in "omics" technologies can be envisioned: 1) immediate, short-term training in the form of workshops, and 2) long-term, comprehensive instruction in the form of graduate education programs. The current proposal aims to fill the former need by providing hands-on training in genomics and proteomics in a coherent, experimentally-based framework. The Directors of the proposal have developed and taught courses covering Proteomics, Genomics, Bioinformatics, and Biostatistics and the curriculum for the proposed course will merge their expertise to provide a comprehensive training overview of these key technologies. Specifically, we propose to accomplish the following: build a curriculum enabling outcome-structured learning in proteomics and genomics technologies, provide novel training experiences using hands-on techniques and an established model by experienced investigators and educators, disseminate educational materials such that knowledge and information can be used to benefit a wide variety of audiences, and provide and/or act as portal for collaborative opportunities for both young and established investigators. (End of Abstract)