STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE SFID REGION OF ESCHERICHIA COLI
STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE SFID REGION OF ESCHERICHIA COLI
批准号:
6271791
负责人:
ANN A MCPARTLAND
金额:
$9.19万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-06-01 至 1999-05-31
中文摘要
本项目的总体目标是探索调节细胞的机制
英文摘要
The overall aim of this project is to probe the mechanisms regulating cell
division in Escherichia coli. The proposed work focuses on the cellular
role of the sfiD gene. Mutations at this locus prevent the action of two
division inhibitors, Su1A and SfiC, whose synthesis is stimulated as part
of the recA-dependent response to DNA damage. Su1A prevents "FtsZ ring
formation," i.e. aggregation of the FtsZ cell division initiator protein
on the cell envelope just prior to division. An sfiD mutation allows FtsZ
ring formation in the presence of Su1A. The sfiD gene is located
between kilobase pair 3,102 and 3,110 on the E. coli physical map, or
roughly 63.7 minutes on the genetic map. A second phenotype associated
with the sfiD mutations is extremely poor growth at temperatures below 29
degree. This "cold sensitivity" may be attributable to partial cell lysis
at low temperature. In complementation studies, the mutant sfiD alleles
are recessive to sfid plus. A number of the proposed experiments are
directed toward structural analysis of the sfiD region. These include
subcloning of complete and partial sfiD gene segments into phagemid
vectors and DNA sequence determination. The nucleotide positions of
mutant sfiD25 and sfiD27 alleles will be established using a combination
of PCR, gene cloning and sequencing. In other experiments the effect of
mutations in sfiD on the integrity of the cell wall will be examined,
either directly or by response to antibiotic inhibitors of peptidoglycan
synthesis. As a means of identifying genes whose products may interact
with sfiD, the map positions of second site suppressor mutations that
reverse the cold sensitivity phenotype will be determined. Finally,
additional experiments designed to explore the physiological role of SfiD
will include monitoring the effect of SfiD overproduction on division
frequency and Su1A stability measurements in sfiD plus versus sfiD mutant
strains.
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STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE SFID REGION OF ESCHERICHIA COLI
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批准号:6335038
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项目类别:
-
资助金额:$10.36万
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财政年份:2000
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负责人:ANN A MCPARTLAND
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依托单位:
STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE SFID REGION OF ESCHERICHIA COLI
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批准号:6107636
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项目类别:
-
资助金额:$10.36万
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财政年份:1999
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负责人:ANN A MCPARTLAND
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依托单位:
STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE SFID REGION OF ESCHERICHIA COLI
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批准号:6217827
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项目类别:
-
资助金额:$10.36万
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财政年份:1999
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负责人:ANN A MCPARTLAND
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依托单位:
STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE SFID REGION OF ESCHERICHIA COLI
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批准号:6240542
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项目类别:
-
资助金额:$9.55万
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财政年份:1997
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负责人:ANN A MCPARTLAND
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依托单位:
海外基金