PURCHASE OF A QUADRUPOLE ION TRAP MASS SPECTROMETER

购买四极杆离子阱质谱仪

• 批准号: 2516739
• 负责人: DAVID W. SPEICHER
• 金额: $ 22.69万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 1999-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2516739
• 关键词: biomedical equipment; biomedical equipment purchase; electrospray ionization mass spectrometry; gas chromatography mass spectrometry; high performance liquid chromatography

Funds are requested to purchase a system consisting of a Finnigan LCQ benchtop mass spectrometer and a capillary HPLC, which will be located in the Wistar Protein Microchemistry/Mass Spectrometry Core Facility. There is currently no mass spectrometer at the Wistar Institute capable of on-line MS and MS-MS analyses. The requested system will complement an existing MALDI mass spectrometer that has proven to be invaluable able for many projects, but is limited by its mass accuracy, its off- line analysis mode, and its more modest capacity to obtain MS-MS data. The requested equipment should especially improve our ability to identify and locate protein posttranslational modifications, and increase our sensitivity when identifying proteins isolated from 1D and 2D gels. The unique analytical capacities of this equipment will benefit at least 23 different laboratories at the Wistar Institute, including a major user group consists of 10 laboratories representing 9 different projects and a minor user group consisting of 13 additional laboratories. All projects involving the major user group and all minor users have NIH grant support. Any additional instrument time, not required by the identified user groups, will be made available to other researchers at the Wistar Institute, the adjacent University Of Pennsylvania, and other institutions with preference given to investigators with NIH funding. An internal advisory committee comprised of all major users and the Wistar Institute Deputy Director will oversee equitable access, operation and administration of the instrument. The Wistar Institute is committed to housing, maintaining and staffing the requested instrument for its useful lifetime as verified by an accompanying letter from the Institute's Director. The Wistar Protein Microchemistry/Mass Spectrometry Core Facility was established by Dr. Speicher in 1986. During the past five years, this core facility has been used by 183 academic laboratories including: 32 of the 40 Wistar laboratories; 81 additional users from 8 other academic institutions in the Philadelphia region; and 70 users from 32 other academic institutions outside the region.

要求提供资金，用于购买一个由Finnigan LCQ 台式质谱仪和毛细管HPLC，将位于 在威斯塔蛋白质微量化学/质谱核心设施中。 目前，Wistar研究所没有质谱仪能够 在线MS和MS-MS分析。 所要求的系统将补充 现有的MALDI质谱仪已被证明是无价的 能够用于许多项目，但受限于其质量精度， 线分析模式，其更适度的能力，以获得MS-MS数据。 所要求的设备应特别提高我们的能力， 鉴定和定位蛋白质翻译后修饰，以及 提高我们在识别从1D分离的蛋白质时的灵敏度， 2D凝胶。 该设备独特的分析能力将 至少有23个不同的实验室受益于Wistar研究所， 包括一个主要用户组，由10个实验室组成， 9个不同的项目和一个由13个额外用户组成的小用户组 laboratories. 所有涉及主要用户组的项目和所有次要用户组的项目 用户有NIH的资助。 任何额外的仪器时间， 所确定的用户组所需的，将提供给其他 邻近的密歇根大学威斯塔研究所的研究人员 宾夕法尼亚州和其他机构，优先考虑 研究人员在NIH的资助下。 内部咨询委员会 由所有主要用户和Wistar研究所副所长组成 将监督公平进入、运作和管理 仪器 威斯塔研究所致力于提供住房， 并在所要求的仪器的使用寿命内为其配备人员， 由研究所所长的附函核实。 的 Wistar蛋白质微化学/质谱核心设施 由Speicher博士于1986年创立。 在过去的五年里，这 核心设施已被183个学术实验室使用，包括： 40个Wistar实验室;来自其他8个学术机构的81个额外用户 费城地区的机构;以及来自32个其他地区的70名用户 区域外的学术机构。