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Purchase of a Q Exactive HF mass spectrometer system for metabolomics

购买用于代谢组学的 Q Exactive HF 质谱仪系统

基本信息

批准号：
9274622
负责人：
DAVID W. SPEICHER
金额：
$ 60万
依托单位：
WISTAR INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-04-14
项目状态：
已结题

项目摘要

PROJECT SUMMARY / ABSTRACT This is a proposal to acquire a Thermo Q Exactive HF mass spectrometer and a conventional flow UPLC for a range of metabolomics assays including untargeted (global) metabolite comparisons, flux analyses, and lipidomics profiling (untargeted and targeted). This instrument is needed to support 8 major users and 2 other users at the Wistar Institute to conduct metabolism and lipidome studies involving 22 NIH grants. Eight of these users have multiple NIH grants that will benefit from the requested mass spectrometer system. One of the other users is Dr. Zachary Schug who does not yet have NIH funding because he recently moved from his postdoctoral position in Scotland to the Wistar Institute. Dr. Schug is a critical contributor to this proposal because he has in-depth expertise in all of the analytical approaches that will be used. It is estimated that this user group will initially require nearly all available time on the requested instrument. However, we expect to improve throughput with experience and anticipate that in future years the number of users will grow substantially, thereby ensuring that this instrument will continue to be heavily utilized. Acquisition of this instrument and expansion of our metabolomics capacity is part of a strong institutional commitment driven by the expanding role of metabolism in multiple aspects of cancer biology and other human diseases, coupled with very limited and inadequate access to these technologies from external sources. As a result, metabolism studies at the Wistar Institute have sometimes not been pursued due to logistical barriers and studies that were pursued sometimes suffered from the lack of adequate consultative expertise to ensure optimal experiment design. The requested system will be housed in the Wistar Institute Proteomics and Metabolomics Core Facility under the supervision of the Facility’s Scientific Director, Dr. David Speicher. Day-to-day maintenance and operation of the instrument will be handled by Dr. Hamid Baniasadi, who trained in Dan Raftery’s lab and has more than 8 years of experience with developing and using metabolomics assays. In addition, Dr. Zachary Schug, who is a recently recruited assistant professor from Eyal Gottlieb’s lab, will provide expert consultative advice and will train and educate users through training workshops, a metabolomics journal club, and individual consultation as needed. The Wistar Institute’s CEO and Cancer Center Director confirms the Institute’s very strong commitment to implementing and maintaining state-of-the-art metabolomics capacities. Wistar has already invested more than $1.6M in metabolomics expertise and capacities. Specific to this grant, the Institute is committed to: 1) providing the $66,571 balance of the purchase price above the SIG cap, 2) covering any costs associated with instrument installation, 3) ensuring support is available for expert operators and instrument maintenance throughout its useful lifetime, and 4) ensuring that NIH funded investigators have primary access to the instrument.

《项目摘要》/《摘要》。 -- 这是一项收购美国Thermo公司、Exactive公司和Exactive公司质谱仪的收购要约，也是一项收购UPLC公司的常规流程的计划，为期一年。代谢物组学分析的范围包括非靶向代谢产物的比对、通量分析、检测和分析。脂肪组学是一种分析工具(非靶向和靶向)。这一工具需要支持8个主要用户和2个其他用户。用户在美国维斯塔尔研究所工作，以便进行代谢研究和脂质组学研究，涉及美国国立卫生研究院的22项研究拨款。这些用户已经获得了多项美国国立卫生研究院的资助，他们将不会从他们要求的质谱仪测试系统中受益。其他用户包括舒格的扎克里·扎克里博士，他还不知道，他已经向NIH提供了资金，因为他最近刚从他的办公室搬了出来。苏格兰博士后职位将被任命为维斯塔尔医学研究所的负责人。他的博士后舒格博士是支持这项新提议的关键贡献者。因为他在所有的分析方法中都有深入的专业知识，所以他不会被使用。但据估计，这一点是不可能的。最初，用户组将要求在请求的仪器上提供几乎所有可用的时间。然而，我们不能指望他们会这样做。以用户体验提升网络吞吐量，并预计未来几年网络用户数量将继续增长。实质上，从而确保了这一金融工具将继续得到大量利用。我们收购了这一工具。我们的代谢组学和产能计划的仪器研发和扩张计划是由中国推动的强大的制度改革承诺的一部分。新陈代谢在癌症、生物学和其他常见人类疾病等多个方面的作用日益扩大，再加上。由于资源非常有限，获得这些新技术的渠道也不充分，这些技术只能从外部来源获得。因此，他们的新陈代谢。在维斯塔尔国际研究所进行的研究有时由于后勤方面的障碍而没有被继续进行，而其他研究也是如此。被追求的人有时会因为缺乏足够的咨询和专业知识而遭受痛苦，以确保最优的实验。设计。我们要求的系统将被安置在维斯塔尔蛋白质组学研究所和新陈代谢组学研究所的核心实验室。该设施由国家科学研究中心主任大卫·斯皮克尔博士负责监督，并进行日常维护。而仪器的操作将由曾接受过丹·哈米德·巴尼亚萨迪医生和拉夫特里的医疗实验室培训的医生负责。拥有超过8年的研究经验，包括药物开发和使用药物代谢组学分析。此外，Zachary博士还说。舒格是一名专家，最近从埃亚尔·戈特利布的实验室招聘了一名助理教授，他将提供专家和咨询。咨询服务和建议将通过培训和研讨会对患者进行培训和教育，并在代谢组学杂志和俱乐部、网站和网站上提供建议。个人可以根据需要进行咨询。美国维斯塔尔癌症研究所首席执行官兼全球癌症研究中心主任证实了这一消息。该研究所非常坚定的承诺是继续实施这项研究，并保持其最先进的代谢组学能力。 Wistar基金会已经在代谢组学、专业知识和研究能力方面投资了超过160万美元。提供这笔赠款的具体资金是什么。该研究所致力于：(1)为高于SIG上限的收购价格提供66,571美元的余额；(2) 承担与仪器安装相关的任何成本，确保专家和操作员能够获得技术支持。在其有用的整个生命周期内，仪器和设备的维护费用确保了NIH资助的费用和调查人员的费用。主要用户可以访问该仪器。