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Purchase of a Q Exactive HF mass spectrometer system for metabolomics

购买用于代谢组学的 Q Exactive HF 质谱仪系统

基本信息

批准号：
9274622
负责人：
DAVID W. SPEICHER
金额：
$ 60万
依托单位：
WISTAR INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-04-14
项目状态：
已结题

项目摘要

PROJECT SUMMARY / ABSTRACT This is a proposal to acquire a Thermo Q Exactive HF mass spectrometer and a conventional flow UPLC for a range of metabolomics assays including untargeted (global) metabolite comparisons, flux analyses, and lipidomics profiling (untargeted and targeted). This instrument is needed to support 8 major users and 2 other users at the Wistar Institute to conduct metabolism and lipidome studies involving 22 NIH grants. Eight of these users have multiple NIH grants that will benefit from the requested mass spectrometer system. One of the other users is Dr. Zachary Schug who does not yet have NIH funding because he recently moved from his postdoctoral position in Scotland to the Wistar Institute. Dr. Schug is a critical contributor to this proposal because he has in-depth expertise in all of the analytical approaches that will be used. It is estimated that this user group will initially require nearly all available time on the requested instrument. However, we expect to improve throughput with experience and anticipate that in future years the number of users will grow substantially, thereby ensuring that this instrument will continue to be heavily utilized. Acquisition of this instrument and expansion of our metabolomics capacity is part of a strong institutional commitment driven by the expanding role of metabolism in multiple aspects of cancer biology and other human diseases, coupled with very limited and inadequate access to these technologies from external sources. As a result, metabolism studies at the Wistar Institute have sometimes not been pursued due to logistical barriers and studies that were pursued sometimes suffered from the lack of adequate consultative expertise to ensure optimal experiment design. The requested system will be housed in the Wistar Institute Proteomics and Metabolomics Core Facility under the supervision of the Facility’s Scientific Director, Dr. David Speicher. Day-to-day maintenance and operation of the instrument will be handled by Dr. Hamid Baniasadi, who trained in Dan Raftery’s lab and has more than 8 years of experience with developing and using metabolomics assays. In addition, Dr. Zachary Schug, who is a recently recruited assistant professor from Eyal Gottlieb’s lab, will provide expert consultative advice and will train and educate users through training workshops, a metabolomics journal club, and individual consultation as needed. The Wistar Institute’s CEO and Cancer Center Director confirms the Institute’s very strong commitment to implementing and maintaining state-of-the-art metabolomics capacities. Wistar has already invested more than $1.6M in metabolomics expertise and capacities. Specific to this grant, the Institute is committed to: 1) providing the $66,571 balance of the purchase price above the SIG cap, 2) covering any costs associated with instrument installation, 3) ensuring support is available for expert operators and instrument maintenance throughout its useful lifetime, and 4) ensuring that NIH funded investigators have primary access to the instrument.

项目总结/摘要这是一项获得Thermo Q Exactive HF质谱仪和常规流动UPLC的建议，用于一系列代谢组学分析，包括非靶向（全局）代谢物比较、通量分析，以及脂质组学分析（非靶向和靶向）。该仪器需要支持8个主要用户和2个其他用户。在Wistar研究所的用户进行代谢和脂质组研究，涉及22个NIH赠款。这些用户有多个NIH赠款，将受益于所请求的质谱仪系统。另一个用户是Zachary Schug博士，他还没有NIH的资助，因为他最近从他的在苏格兰的威斯塔研究所的博士后职位。Schug博士是这一建议的关键贡献者因为他对所有将要使用的分析方法都有深入的专业知识。据估计，用户组最初将需要在所请求的仪器上使用几乎所有可用时间。但是，我们预计通过经验提高吞吐量，并预计未来几年用户数量将增长因此，这一工具将继续得到大量使用。仪器和扩大我们的代谢组学能力是一个强大的机构承诺的一部分，代谢在癌症生物学和其他人类疾病的多个方面的作用不断扩大，从外部来源获得这些技术的机会非常有限和不足。因此，在Wistar研究所的研究有时没有进行，由于后勤障碍和研究，有时由于缺乏足够的咨询专门知识，无法确保进行最佳试验，所要求的系统将被安置在Wistar研究所蛋白质组学和代谢组学核心在设施科学主任大卫·斯派克博士的监督下进行设施的日常维护。该仪器的操作将由哈米德·巴尼亚萨迪博士负责，他曾在丹·拉夫特里的实验室接受过培训， Zachary博士在开发和使用代谢组学分析方面拥有超过8年的经验。此外，Zachary博士 Schug是Eyal Gottlieb实验室最近招聘的助理教授，他将提供专家咨询，建议，并将通过培训讲习班，代谢组学杂志俱乐部， Wistar研究所的首席执行官和癌症中心主任证实，研究所非常坚定地致力于实施和维持最先进的代谢组学能力。 Wistar已经在代谢组学专业知识和能力方面投入了超过160万美元。具体到这笔赠款，研究所承诺：1）提供高于SIG上限的购买价格的66，571美元余额，2）涵盖与仪器安装相关的任何费用，3）确保为专业操作员提供支持和仪器维护在其整个使用寿命，和4）确保NIH资助的研究人员，第一次进入仪器。