Proteomics Facility

蛋白质组学设施

• 批准号: 7945027
• 负责人: DAVID W. SPEICHER
• 金额: $ 9.25万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-15 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7945027
• 关键词: Acetylation; Amino Acid Sequence; Arts; Biological Assay; Biological Markers; Blood capillaries; Buffers; Cancer Center; Cancer Center Support Grant; Complex; Computer software; Consensus; Consultations; Custom; Data; Data Analyses; Data Set; Databases; Detection; Device or Instrument Development; Digestion; Ensure; Environmental Pollution; Enzymes; Equipment; Fees; Gases; Gel; Genes; Genome; Genomics; Goals; Grant; Growth; Hand; Heterogeneity; High Pressure Liquid Chromatography; Hybrids; Ion Exchange; Ions; Laboratory Research; Liquid substance; Macromolecular Complexes; Manuals; Mass Spectrum Analysis; Measures; Membrane; Methods; Methylation; Modification; N-terminal; Operative Surgical Procedures; Organism; Peer Review; Peptide Hydrolases; Peptide Mapping; Peptide Sequence Determination; Peptides; Performance; Phase; Phosphoproteins; Phosphorylated Peptide; Phosphorylation; Post-Translational Protein Processing; Problem Solving; Procedures; Process; Protein Analysis; Protein Biochemistry; Protein Sequence Analysis; Proteins; Proteome; Proteomics; Protocols documentation; Published Comment; Recombinant Proteins; Relative (related person); Research; Research Personnel; Resolution; Robotics; Salts; Sampling; Scanning; Screening for cancer; Services; Signal Transduction; Silver Staining; Slice; Software Tools; Solutions; Solvents; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; Subcellular Fractions; System; Techniques; Technology; Training; Trypsin; Tyrosine; Update; Variant; air filter; analytical method; base; capillary; chymotrypsin; comparative; cost; crosslink; discount; improved; instrument; instrumentation; interest; mass spectrometer; meetings; member; nano; polyvinylidene fluoride; pressure; programs; protein complex; protein profiling; research study; reversed phase chromatography; success

The Proteomics Facility provides a comprehensive suite of proteomics services to the Cancer Center membership. The primary emphasis is to provide expert consultation and state-of-the-art technologies operating at maximum performance at affordable costs to Cancer Center members. This is quite challenging because unlike genomics, proteomics technologies are highly varied; there are not consensus optimal approaches for most problems, and instruments, software and analytical strategies continue to evolve rapidly. Therefore, equipment and analytical methods must be continually updated. As a result, most of the instruments in the Facility were purchased within the past grant period, which includes $1.3 million invested in 2008 alone to replace obsolete mass spectrometers with 2 LTQ Orbitrap mass spectrometers and a high sensitivity triple quadropole mass spectrometer. Sensitivity of protein identifications has increased more than 100-fold over the past grant period through a combination of method optimization, acquisition of more sensitive instruments and development of an improved data analysis pipeline. Other methods have been similarly improved and new complementary methods have been implemented. The facility is also beginning to implement quantitative protein profile comparisons on LC-MS/MS based analyses using several complementary approaches, which will be expanded during the next grant period. During the past grant period (2003-present), 19 CC members used the Proteomics Facility and additional members benefited because many projects were collaborative between multiple CC investigators. CC members receive priority in sample analysis, substantial discounts on user fees (35-50% depending upon the service), more extensive consultation, training and assistance, and early access to methods/technologies not yet implemented as routine services. In the current year CCSG support covered 27% of non-equipment operating costs and will cover 23% of operating costs during the first year of the proposed grant. During 2007 44% of facility usage was by peer-reviewed CC members, and over the entire past grant period (2003-present) 61% of facility usage was by peer-reviewed CC members.

蛋白质组学设施为癌症中心提供一套全面的蛋白质组学服务 会员资格主要重点是提供专家咨询和最先进的技术 以最高的性能和可承受的成本为癌症中心成员提供服务。这是相当具有挑战性的 因为与基因组学不同，蛋白质组学技术是高度多样化的; 解决大多数问题的方法、工具、软件和分析战略继续发展 迅速因此，设备和分析方法必须不断更新。因此，大多数 该基金中的工具是在过去的赠款期内购买的，其中包括130万美元的投资 仅在2008年，就用2台LTQ Orbitrap质谱仪和一台更高的 灵敏度三重四极杆质谱仪蛋白质鉴定的灵敏度提高了更多 通过优化组合方式，收购量超过以往100倍， 敏感仪器和开发改进的数据分析管道。其他方法已经 已采用类似的改进方法和新的补充方法。该设施也开始 为了使用几种基于LC-MS/MS的分析进行定量蛋白质谱比较， 补充办法，将在下一个赠款期间扩大。在过去的赠款 2003年至今，19个CC成员使用了蛋白质组学设施，更多的成员受益 因为许多项目是多个CC调查员之间的合作。CC会员优先 在样本分析中，用户费用的大幅折扣（35-50%，取决于服务），更广泛的 咨询、培训和援助，以及抢先体验尚未实施的方法/技术， 例行服务。在本年度，CCSG的支助占非设备业务费用的27%， 在建议拨款的第一年支付23%的运作成本。2007年，44%的设施使用率 是由同行评审的CC成员，在整个过去的赠款期间（2003年至今）61%的设施 由同行评审的CC成员使用。