Proteomics Facility

蛋白质组学设施

• 批准号: 7945027
• 负责人: DAVID W. SPEICHER
• 金额: $ 9.25万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-15 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7945027
• 关键词: Acetylation; Amino Acid Sequence; Arts; Biological Assay; Biological Markers; Blood capillaries; Buffers; Cancer Center; Cancer Center Support Grant; Complex; Computer software; Consensus; Consultations; Custom; Data; Data Analyses; Data Set; Databases; Detection; Device or Instrument Development; Digestion; Ensure; Environmental Pollution; Enzymes; Equipment; Fees; Gases; Gel; Genes; Genome; Genomics; Goals; Grant; Growth; Hand; Heterogeneity; High Pressure Liquid Chromatography; Hybrids; Ion Exchange; Ions; Laboratory Research; Liquid substance; Macromolecular Complexes; Manuals; Mass Spectrum Analysis; Measures; Membrane; Methods; Methylation; Modification; N-terminal; Operative Surgical Procedures; Organism; Peer Review; Peptide Hydrolases; Peptide Mapping; Peptide Sequence Determination; Peptides; Performance; Phase; Phosphoproteins; Phosphorylated Peptide; Phosphorylation; Post-Translational Protein Processing; Problem Solving; Procedures; Process; Protein Analysis; Protein Biochemistry; Protein Sequence Analysis; Proteins; Proteome; Proteomics; Protocols documentation; Published Comment; Recombinant Proteins; Relative (related person); Research; Research Personnel; Resolution; Robotics; Salts; Sampling; Scanning; Screening for cancer; Services; Signal Transduction; Silver Staining; Slice; Software Tools; Solutions; Solvents; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; Subcellular Fractions; System; Techniques; Technology; Training; Trypsin; Tyrosine; Update; Variant; air filter; analytical method; base; capillary; chymotrypsin; comparative; cost; crosslink; discount; improved; instrument; instrumentation; interest; mass spectrometer; meetings; member; nano; polyvinylidene fluoride; pressure; programs; protein complex; protein profiling; research study; reversed phase chromatography; success

The Proteomics Facility provides a comprehensive suite of proteomics services to the Cancer Center membership. The primary emphasis is to provide expert consultation and state-of-the-art technologies operating at maximum performance at affordable costs to Cancer Center members. This is quite challenging because unlike genomics, proteomics technologies are highly varied; there are not consensus optimal approaches for most problems, and instruments, software and analytical strategies continue to evolve rapidly. Therefore, equipment and analytical methods must be continually updated. As a result, most of the instruments in the Facility were purchased within the past grant period, which includes $1.3 million invested in 2008 alone to replace obsolete mass spectrometers with 2 LTQ Orbitrap mass spectrometers and a high sensitivity triple quadropole mass spectrometer. Sensitivity of protein identifications has increased more than 100-fold over the past grant period through a combination of method optimization, acquisition of more sensitive instruments and development of an improved data analysis pipeline. Other methods have been similarly improved and new complementary methods have been implemented. The facility is also beginning to implement quantitative protein profile comparisons on LC-MS/MS based analyses using several complementary approaches, which will be expanded during the next grant period. During the past grant period (2003-present), 19 CC members used the Proteomics Facility and additional members benefited because many projects were collaborative between multiple CC investigators. CC members receive priority in sample analysis, substantial discounts on user fees (35-50% depending upon the service), more extensive consultation, training and assistance, and early access to methods/technologies not yet implemented as routine services. In the current year CCSG support covered 27% of non-equipment operating costs and will cover 23% of operating costs during the first year of the proposed grant. During 2007 44% of facility usage was by peer-reviewed CC members, and over the entire past grant period (2003-present) 61% of facility usage was by peer-reviewed CC members.

蛋白质组学设施为癌症中心提供一整套蛋白质组学服务 会员制。主要的重点是提供专家咨询和最先进的技术 以癌症中心成员负担得起的成本以最高的性能运营。这是相当具有挑战性的 因为与基因组学不同，蛋白质组学技术是多种多样的；没有共识是最优的 解决大多数问题的方法以及工具、软件和分析策略都在继续发展 迅速地。因此，设备和分析方法必须不断更新。因此，大多数 该机制的工具是在过去的赠款期间购买的，其中包括130万美元的投资 仅在2008年就用2台LTQ Orbitrap质谱仪和1台高质量质谱仪取代了过时的质谱仪 灵敏度三重四极质谱计。蛋白质鉴定的灵敏度提高了更多 在过去的授权期内通过组合方法优化100倍以上，收购了 敏感的仪器和改进的数据分析管道的开发。其他方法包括 同样，已经实施了改进和新的补充方法。该设施也正在开始 在基于LC-MS/MS的分析中使用几种 补充办法，将在下一个赠款期间扩大。在过去的拨款期间 在此期间(2003年至今)，19个CC成员使用了蛋白质组学机制，更多的成员受益 因为许多项目是多个CC调查人员之间的协作。CC会员可享有优先权 在样本分析中，用户费用的大幅折扣(35%-50%，取决于服务)，更广泛 协商、培训和援助以及及早获得尚未实施的方法/技术 例行服务。在本年度，CCSG的支持覆盖了27%的非设备运营成本，并将 在拟议拨款的第一年支付23%的运营成本。2007年，44%的设施使用率 是由经同行审查的CC成员提供的，在过去整个赠款期间(2003年至今)，61%的贷款 使用情况由经同行审查的CC成员使用。